Razin S, Gross M, Wormser M, Pollack Y, Glaser G
In Vitro. 1984 May;20(5):404-8. doi: 10.1007/BF02619586.
Infection of cell cultures by mycoplasmas can be detected and the mycoplasma identified by Southern blot hybridization of the Eco RI-digested DNA of the suspected cell cultures with a nick-translated probe consisting of cloned ribosomal RNA genes of Mycoplasma capricolum. The probe does not hybridize with eukaryotic DNA. The hybridization pattern with mycoplasmal DNA is species specific, enabling the identification of the four most prevalent mycoplasma contaminants, Mycoplasma orale, Mycoplasma hyorhinis, Mycoplasma arginini, and Acholeplasma laidlawii. The test is also very sensitive and can detect as little as 1 ng of mycoplasmal DNA, roughly equivalent to the DNA content of 10(5) mycoplasmas.
通过用由山羊支原体核糖体RNA基因克隆组成的切口平移探针，对疑似细胞培养物经Eco RI酶切的DNA进行Southern印迹杂交，可检测细胞培养物中的支原体感染并鉴定支原体。该探针不与真核DNA杂交。支原体DNA的杂交模式具有种属特异性，能够鉴定出四种最常见的支原体污染物，即口腔支原体、猪鼻支原体、精氨酸支原体和莱氏无胆甾原体。该检测也非常灵敏，能够检测低至1 ng的支原体DNA，大致相当于10⁵个支原体的DNA含量。
