Institute of Livestock and Grassland Science, National Agricultural and Food Research Organization, Ibaraki 305-0901, Japan.
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005278.
The genus plays an important role in polysaccharide degradation and fermentation in the rumen. To further understand the function of the phylogenetically diverse genus , it is necessary to explore the individual characteristics at the species level. In this study, Gram-negative anaerobic bacterial strains isolated from the rumen of Holstein cows were identified. Strain R5019 was classified within the genus based on 16S rRNA gene sequence-based phylogenetic analysis. The values of 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization between strain R5019 and its phylogenetically nearest species PPPA20 were 89.8, 82.6, and 29.3 %, respectively. The genome size of R5019 was estimated to be ca. 4.19 Mb with a genomic G+C content of 49.5 mol%. The major cellular fatty acids and menaquinones were C anteiso and C anteiso and MK-11 and MK-12, respectively. Succinate, lactate, malate, acetate and formate were produced as the fermentation end products using glucose. Based on phylogenetic, physiological, biochemical and genomic differences between 11 strains and other phylogenetically related species, a novel species, sp. nov., is proposed within the genus . The type strain is R5019 (=JCM 34664=DSM 112675).
该属在反刍动物的多糖降解和发酵中起着重要作用。为了进一步了解在系统发育上多样化的属的功能，有必要在种的水平上探索其个体特征。本研究从荷斯坦奶牛瘤胃中分离出革兰氏阴性厌氧细菌菌株，并对其进行了鉴定。根据 16S rRNA 基因序列的系统发育分析，菌株 R5019 被归类为属。菌株 R5019 与系统发育上最接近的种 PPPA20 的 16S rRNA 基因序列相似性、平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 89.8%、82.6%和 29.3%。R5019 的基因组大小估计约为 4.19Mb，基因组 G+C 含量为 49.5mol%。主要的细胞脂肪酸和menaquinones 分别为 C anteiso 和 C anteiso 和 MK-11 和 MK-12。利用葡萄糖作为发酵终产物产生琥珀酸盐、乳酸盐、苹果酸盐、醋酸盐和甲酸盐。基于 11 株菌和其他系统发育上相关种之间的系统发育、生理、生化和基因组差异，提出了一个新的种，即 。该模式株为 R5019（=JCM 34664=DSM 112675）。
