羟基磷灰石混悬液对原位 48 小时牙菌斑形成的影响。
Influence of a hydroxyapatite suspension on 48-h dental biofilm formation in-situ.
机构信息
Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421, Homburg, Saar, Germany.
Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, D-66421, Homburg, Saar, Germany.
出版信息
Arch Oral Biol. 2022 Apr;136:105388. doi: 10.1016/j.archoralbio.2022.105388. Epub 2022 Feb 26.
OBJECTIVE
The aim of the present in-situ study was to investigate anti-adherent properties of mouthrinses containing hydroxyapatite (HAP) nanoparticles on oral biofilm formation.
DESIGN
Biofilm was formed for 48 h on bovine enamel or dentine specimens that were fixed to maxillary splints and worn intraoral by six volunteers. During biofilm formation, rinsing was performed with sterile water, HAP (5%) or chlorhexidine (0.2%) according to two different rinsing protocols in order to assess substantivity. Scanning electron microscopy was used to investigate biofilm coverage of specimens, biofilm thickness and morphology. In addition, saliva samples were collected and analysed with transmission electron microscopy.
RESULTS
Rinsing with sterile water or HAP resulted in 2.1 or 2.3 µm thick biofilms, respectively, covering more than half of specimen' surfaces. Despite single deposits of nanoparticles in saliva and biofilm, HAP did not inhibit biofilm formation. Chlorhexidine on the other hand significantly reduced biofilm thickness and coverage.
CONCLUSIONS
Mouthrinses containing HAP nanoparticles showed no anti-adherent effects during 48 h of biofilm formation in-situ.
目的
本原位研究旨在研究含纳米羟基磷灰石（HAP）的漱口水对口腔生物膜形成的抗附着特性。
设计
将牛牙釉质或牙本质标本固定在上颌夹板上，并由六名志愿者佩戴在口腔内 48 小时形成生物膜。在生物膜形成过程中，根据两种不同的冲洗方案，用无菌水、HAP（5%）或洗必泰（0.2%）冲洗，以评估持久性。扫描电子显微镜用于研究标本上的生物膜覆盖范围、生物膜厚度和形态。此外，收集唾液样本并通过透射电子显微镜进行分析。
结果
用无菌水或 HAP 冲洗后，生物膜的厚度分别为 2.1 或 2.3 µm，覆盖了标本表面的一半以上。尽管唾液和生物膜中存在纳米颗粒的单个沉积物，但 HAP 并没有抑制生物膜的形成。另一方面，洗必泰显著降低了生物膜的厚度和覆盖率。
结论
在原位 48 小时生物膜形成期间，含 HAP 纳米颗粒的漱口水没有表现出抗附着作用。
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