The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Yan'an Road 395, 310006, Hangzhou, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, Kaixuan Road 268, 310020, Hangzhou, China; Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Building 73, Saarland University, D-66421, Homburg, Germany.
Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Building 73, Saarland University, D-66421, Homburg, Germany.
J Dent. 2020 Nov;102:103482. doi: 10.1016/j.jdent.2020.103482. Epub 2020 Sep 24.
To investigate the effects of Chinese gallnut extracts and pure tannic acid on in situ biofilm formation on enamel and dentin samples over 24 h.
Bovine enamel and dentin samples were buccally fixed on maxillary splints. Six volunteers wore the splints for 24 h, and rinsed their mouths with tap water (control), 1% tannic acid- and 1% Chinese gallnut extracts-containing solution twice a day, 3 min after the splints were placed in the mouth and before night sleep. Live/dead staining was used for fluorescence microscopic (FM) visualization and quantification of bacteria viability of biofilms formed on enamel and dentin samples. Biofilm coverage was evaluated and recorded by FM and scanning electron microscopy (SEM). In addition, biofilms were analyzed by transmission electron microscopy (TEM). The Kruskal-Wallis test was used to analyze biofilm data.
Rinsing with tannic acid- and Chinese gallnut extracts-containing solutions significantly reduced in situ biofilm coverage on enamel and dentin samples (P < 0.05). The bacterial viability of biofilms formed on enamel samples was significantly reduced compared to the control (P < 0.05). TEM analysis revealed an increase in pellicle's electron density and thickness and only few or no bacteria adherent to the pellicle in the experimental samples.
Rinsing with tannic acid- and Chinese gallnut extracts-containing solutions can effectively inhibit in situ biofilm formation, modify the ultrastructure of biofilms on enamel and dentin surfaces and significantly reduce the bacterial viability of biofilm on enamel surfaces.
Tannic acid- and Chinese gallnut extracts-containing solutions might be used for dental biofilm management.
研究五倍子提取物和单宁酸纯品对牙釉质和牙本质表面原位生物膜形成的影响，观察时间为 24 小时。
将牛牙釉质和牙本质样本固定在上颌夹板的颊侧。6 名志愿者佩戴夹板 24 小时，每天用自来水（对照组）、含 1%单宁酸和 1%五倍子提取物的溶液漱口两次，在将夹板放入口腔后 3 分钟和睡前各一次。使用活/死染色荧光显微镜（FM）可视化和量化牙釉质和牙本质样本上形成的生物膜中的细菌活力。使用 FM 和扫描电子显微镜（SEM）评估和记录生物膜的覆盖范围。此外，还通过透射电子显微镜（TEM）对生物膜进行分析。使用 Kruskal-Wallis 检验分析生物膜数据。
用含单宁酸和五倍子提取物的溶液漱口显著降低了牙釉质和牙本质样本上的原位生物膜覆盖率（P<0.05）。与对照组相比，牙釉质样本上形成的生物膜中的细菌活力显著降低（P<0.05）。TEM 分析显示，牙菌斑的电子密度和厚度增加，实验样本中只有少数或没有细菌附着在牙菌斑上。
用含单宁酸和五倍子提取物的溶液漱口可以有效抑制原位生物膜形成，改变牙釉质和牙本质表面生物膜的超微结构，显著降低牙釉质表面生物膜的细菌活力。
含单宁酸和五倍子提取物的溶液可能用于口腔生物膜管理。
