Structure and Candidate Biosynthetic Gene Cluster of a Manumycin-Type Metabolite from .

A new manumycin-type natural product named pacificamide () and its candidate biosynthetic gene cluster () were discovered from the marine actinobacterium CNT-855. The structure of the compound was determined using NMR, electronic circular dichroism, and bioinformatic predictions. The gene cluster is unique to and found in only two of the 119 genomes analyzed across nine species. Comparative analyses of biosynthetic gene clusters encoding the production of related manumycin-type compounds revealed genetic differences in accordance with the unique pacificamide structure. Further queries of manumycin-type gene clusters from public databases revealed their limited distribution across the phylum Actinobacteria and orphan diversity that suggests additional products remain to be discovered in this compound class. Production of the known metabolite triacsin D is also reported for the first time from the genus . This study adds two classes of compounds to the natural product collective isolated from the genus , which has proven to be a useful model for natural product research.