Discipline of Histology and Regenerative Dentistry, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Laboratory of Radiobiology, Victor Babeş National Institute of Pathology, Bucharest, Romania;
Rom J Morphol Embryol. 2021 Jul-Sep;62(3):785-792. doi: 10.47162/RJME.62.3.16.
This study aimed to assess the in vitro biocompatibility of titanium (Ti) alloy orthodontic mini-implants by correlating human osteoblasts (HOb) response with chemical composition and surface morphology of mini-implants. HOb were cultivated with or without custom-made and commercial mini-implants, discs and filings. The surface morphology and chemical composition of the implants were assessed under the scanning electron microscopy (SEM) with energy-dispersive X-ray (EDX) microanalysis system. Cell viability, adhesion and proliferation were analyzed by optical microscopy and flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reduction and lactate dehydrogenase (LDH) release tests were used to assess the cytotoxicity of discs and filings-treated culture medium. Shape, adhesion, and multiplication of HOb were not significantly altered by the presence of mini-implants, discs or filings in culture, even though Ti alloy may exert in vitro a low cytotoxic effect on HOb adhered to discs. Morphology analysis by SEM demonstrated that custom-made mini-implants' surface differs from that of commercial mini-screws in terms of surface finish and roughness, whilst EDX analysis showed largely similar percentages of Ti, aluminum and vanadium for the two types of implants. No major differences were noticed regarding the effect exerted in vitro on HOb by the investigated implants. The new mini-implants have a convenient in vitro cytotoxicity profile on HOb.
本研究旨在通过将人成骨细胞（HOb）的反应与迷你种植体的化学成分和表面形态相关联，来评估钛（Ti）合金正畸迷你种植体的体外生物相容性。将 HOb 与定制和商业迷你种植体、圆盘和锉刀一起或不一起培养。使用扫描电子显微镜（SEM）和能量色散 X 射线（EDX）微分析系统评估种植体的表面形态和化学成分。通过光学显微镜和流式细胞术分析细胞活力、粘附和增殖。使用 3-（4,5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑（MTS）还原和乳酸脱氢酶（LDH）释放试验评估圆盘和锉刀处理培养基的细胞毒性。即使 Ti 合金可能对附着在圆盘上的 HOb 产生体外低细胞毒性作用，但迷你种植体、圆盘或锉刀在培养物中存在并不会显著改变 HOb 的形状、粘附和增殖。SEM 形态分析表明，定制迷你种植体的表面在表面光洁度和粗糙度方面与商业迷你螺丝不同，而 EDX 分析表明两种类型的种植体中 Ti、铝和钒的百分比大致相似。在所研究的植入物对 HOb 体外发挥的作用方面，没有发现明显差异。新型迷你种植体对 HOb 具有方便的体外细胞毒性特征。
