Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, P.O. Box: 5166614779, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Sci Rep. 2022 Mar 9;12(1):3878. doi: 10.1038/s41598-022-07845-6.
This study aimed to investigate the prevalence of foodborne pathogenic bacteria in bovine milk, their antibiogram phenotype, and the carriage of antibiotic resistance genes. Raw bovine milk samples (n = 100) were randomly collected from different suppliers in the northwest of Iran. Antibiotic-resistant patterns and the presence of antibiotic resistance genes were evaluated in the isolates. Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. were isolated from 78%, 47%, 25%, and 21% of samples, respectively. All isolates showed high rates of resistance to amoxicillin, penicillin, and cefalexin. The bla and bla genes were detected in 50.0% and 6.4% of E. coli isolates, respectively. Also, 28.5% and 19.0% of Salmonella isolates were positive for bla and bla. The frequency of mecA and bla in S. aureus isolates was 20.0% and 12.0%, respectively. The high prevalence of bovine milk contamination with antimicrobial-resistant species in this study necessitates precise control on antibiotic prescription in veterinary medicine.
本研究旨在调查食源性致病菌在牛初乳中的流行情况、它们的抗生素表型以及携带的抗生素耐药基因。从伊朗西北部的不同供应商处随机采集了 100 份原始牛初乳样本。评估了分离物中的抗生素耐药模式和抗生素耐药基因的存在情况。分别从 78%、47%、25%和 21%的样本中分离出大肠杆菌、单核细胞增生李斯特菌、金黄色葡萄球菌和沙门氏菌。所有分离株对阿莫西林、青霉素和头孢氨苄的耐药率均较高。bla 和 bla 基因分别在 50.0%和 6.4%的大肠杆菌分离株中被检出。此外，28.5%和 19.0%的沙门氏菌分离株对 bla 和 bla 呈阳性。金黄色葡萄球菌分离株的 mecA 和 bla 的检出率分别为 20.0%和 12.0%。本研究中牛初乳中存在大量对抗菌药物耐药的物种，这需要对兽医中抗生素的使用进行精确控制。
