Assistant, Department of Physiology and Anatomy, Institute of Biology and Biomedicine; National Research Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950, Russia.
PhD Student, Department of Neurotechnologies; National Research Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950, Russia.
Sovrem Tekhnologii Med. 2021;13(6):36-41. doi: 10.17691/stm2021.13.6.04. Epub 2021 Dec 28.
is to identify the mechanisms mediating differences in sexual behavior between Sprague Dawley and Wistar rats, in order to choose the optimal stock for research into pharmacological correction of male sexual dysfunction.
The experiments were carried out on sexually mature male rats of two stocks (Sprague Dawley and Wistar) weighing 350-450 g and aged 3 to 6 months. The comparative study of animal behavior was performed using standard tests for social interaction, locomotor activity, and anxiety level, as well as male mating behavior patterns. In order to determine the role of hypothalamic glycine receptors in the male sexual behavior, pharmacological manipulations of glycine receptor activity during mating with receptive females were conducted via bilateral intracerebral microcannulas implanted in the medial preoptic area (mPOA) of the male rat anterior hypothalamus.
The obtained results revealed statistically significant inter-stock differences in sexual behavior at the final consummatory stage of both intact animals and those after pharmacological activation of glycine receptors in the mPOA. The number of anxiety-related grooming patterns in the Open Field test significantly differed between the stocks for both intact animals and those after pharmacological activation of glycine receptors; the observed differences disappeared after the mPOA glycine receptors were blocked. In the Crowley test of social interaction, no significant difference was found between the stocks.Thus, the revealed difference in sexual behavior between Sprague Dawley and Wistar male rats is likely due to the difference in the level of anxiety, which, in turn, may be associated with difference in the mechanisms of glycinergic neurotransmission in the hypothalamic mPOAs of these rats.
To study the relationship between the level of anxiety and sexual behavior, the choice of the Wistar rat stock is optimal since the male sexual behavior in this stock is more sensitive to stress than that in Sprague Dawley rats. However, to model male sexual dysfunction not associated with anxiety, the use of Sprague Dawley male rats should be preferred as these animals show more stable sexual behavior, which is less dependent on the level of anxiety.
目的是确定介导 Sprague Dawley 和 Wistar 大鼠性行为差异的机制，以便选择用于研究药物纠正男性性功能障碍的最佳品系。
实验在两种品系（Sprague Dawley 和 Wistar）的成熟雄性大鼠（体重 350-450 克，年龄 3-6 个月）上进行。使用社交互动、运动活性和焦虑水平的标准测试以及雄性交配行为模式对动物行为进行比较研究。为了确定下丘脑甘氨酸受体在雄性性行为中的作用，通过双侧脑内微插管在雄性大鼠下丘脑前内侧视前区（mPOA）进行甘氨酸受体活性的药理学操作，进行了与接受雌性的交配。
在完整动物和 mPOA 中甘氨酸受体药理学激活后的动物的最终完成阶段，获得的结果显示性行为存在统计学上显著的种间差异。在开放式试验中，与完整动物和 mPOA 中甘氨酸受体药理学激活后的动物相比，焦虑相关梳理模式的数量在品系之间存在显著差异；在 mPOA 甘氨酸受体被阻断后，观察到的差异消失。在 Crowley 社交互动测试中，品系之间没有发现显著差异。因此，Sprague Dawley 和 Wistar 雄性大鼠之间性行为的差异可能归因于焦虑水平的差异，而焦虑水平的差异又可能与这些大鼠下丘脑 mPOA 中的甘氨酸能神经传递机制的差异有关。
为了研究焦虑水平与性行为之间的关系，选择 Wistar 大鼠品系是最优的，因为该品系的雄性性行为对压力比 Sprague Dawley 大鼠更为敏感。然而，为了模拟与焦虑无关的男性性功能障碍，应优先使用 Sprague Dawley 雄性大鼠，因为这些动物表现出更稳定的性行为，对焦虑水平的依赖性较小。
