Research Scholar, School of Forensic Science and Risk Management; Rashtriya Raksha University, Raksha Shakti Rd., Lavad, Gandhinagar, Gujarat, 382305, India.
Lecturer, Jindal Institute of Behavioural Sciences; O.P. Jindal Global University, Sonipat Narela Road, Near Jagdishpur Village, Sonipat, Haryana, 131001, India.
Sovrem Tekhnologii Med. 2021;13(6):43-55. doi: 10.17691/stm2021.13.6.05. Epub 2021 Dec 28.
was to analyze unrelated samples from the Rewa male population of Central India by targeting Y-short tandem repeats (STR) and then compare the results to previously published Y-STR haplotype data.
A total of 181 unrelated male subjects from the Rewa population were genotyped for seventeen Y-STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4) by using an AmpFlSTR® Yfiler™ multiplex kit (Thermo Fisher Scientific, USA). The allele frequencies and forensic parameters were evaluated.
A total of 111 distinct Y-STR alleles with corresponding frequencies ranging from 0.006 to 0.829 were identified. The gene diversity values ranged from 0.3092 at DYS437 to 0.8188 at DYS385b. The studied population showed a high level of haplotype diversity (0.9985) and discrimination capacity (0.927). A haplotype analysis was also conducted. Among the 181 unrelated male samples, 165 haplotypes and 153 unique haplotypes were found. Additionally, Rst (genetic distance) values were calculated using the analysis of molecular variance (AMOVA) for the studied population and for other 18 populations described in the literature. The Rst provides a convenient parameter for estimating the level of genetic differentiation from the microsatellite data. Based on these Rst values and using the multidimensional scaling plot, a neighbor-joining tree was constructed.
The high values of haplotype diversity and discrimination capacity indicate a great potential for distinguishing between male individuals in the studied population. The present population data are expected to find their use in forensic caseworks and population genetics.
分析来自印度中部雷瓦男性群体的无关样本，以靶向 Y 短串联重复序列（STR），然后将结果与先前发表的 Y-STR 单倍型数据进行比较。
对雷瓦群体的 181 名无关男性个体进行了 17 个 Y-STR（DYS19、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS385a/b、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635 和 Y-GATA-H4）的基因分型，使用 AmpFlSTR® Yfiler™ 多重试剂盒（美国赛默飞世尔科技公司）。评估了等位基因频率和法医参数。
共鉴定出 111 个独特的 Y-STR 等位基因，相应的频率范围为 0.006 至 0.829。基因多样性值范围从 DYS437 的 0.3092 到 DYS385b 的 0.8188。研究人群表现出高度的单倍型多样性（0.9985）和鉴别能力（0.927）。还进行了单倍型分析。在 181 名无关男性样本中，发现了 165 种单倍型和 153 种独特单倍型。此外，还使用基于分子方差分析（AMOVA）计算了研究人群和文献中描述的其他 18 个人群的 Rst（遗传距离）值。Rst 为从微卫星数据估计遗传分化水平提供了一个方便的参数。基于这些 Rst 值并使用多维尺度图，构建了一个邻接聚类树。
高的单倍型多样性和鉴别能力值表明，该研究人群中区分个体的潜力很大。本研究人群的数据有望在法医案件和群体遗传学中得到应用。
