Grodetskaya Tatiana A, Evlakov Peter M, Fedorova Olga A, Mikhin Vyacheslav I, Zakharova Olga V, Kolesnikov Evgeny A, Evtushenko Nadezhda A, Gusev Alexander A
Research Institute of Innovative Technologies of the Forestry Complex, Laboratory of PCR Analysis, Voronezh State University of Forestry and Technologies Named after G. F. Morozov, 394087 Voronezh, Russia.
Institute for Environmental Science and Biotechnology, Derzhavin Tambov State University, 392020 Tambov, Russia.
Nanomaterials (Basel). 2022 Mar 4;12(5):864. doi: 10.3390/nano12050864.
Recently, metal oxide nanoparticles (NPs) have attracted attention as promising components for the protection and stimulation of plant microclones in tissue culture in vitro. However, the effect of NPs on the genetic mechanisms underlying plant adaptive responses remains poorly understood. We studied the effect of column-shaped CuO NPs 50 nm in diameter and 70-100 nm in length at a concentration of 0.1-10 mg/L on the development of phytopathogenic fungi , , and in culture, as well as on the infection of downy birch micro-clones with phytopathogens and the level of genes expression associated with the formation of plant responses to stress induced by microorganisms. CuO NPs effectively suppressed the development of colonies of phytopathogenic fungi and (up to 68.42% inhibition at 10 mg/L CuO NPs) but not the development of a colony of . Exposure to the NPs caused multidirectional responses at the level of plant genes transcription: 5 mg/L CuO NPs significantly increased the expression level of the and genes and decreased the expression of and . Infection with significantly increased the level of , , , , and transcripts in birch micro-clones; however, upon exposure to a medium with NPs and simultaneous exposure to a phytopathogen, the expression of the , , and genes decreased by 5.4 times, which is associated with a decrease in the pathogenic load caused by the effect of NPs and the simultaneous stimulation of clones in vitro. The results obtained can be used in the development of preparations based on copper oxide NPs for disinfection and stimulation of plant phytoimmunity during clonal micropropagation of tree crops.
最近，金属氧化物纳米颗粒（NPs）作为在体外组织培养中保护和刺激植物微克隆的有前景的成分而受到关注。然而，NPs对植物适应性反应潜在遗传机制的影响仍知之甚少。我们研究了直径50nm、长度70 - 100nm、浓度为0.1 - 10mg/L的柱状CuO NPs对植物致病真菌、和在培养物中的生长，以及对毛枝桦微克隆被植物病原体感染情况和与植物对微生物诱导的应激反应形成相关的基因表达水平的影响。CuO NPs有效抑制了植物致病真菌和的菌落生长（在10mg/L CuO NPs时抑制率高达68.42%），但对的菌落生长没有抑制作用。暴露于NPs会在植物基因转录水平引起多向反应：5mg/L CuO NPs显著增加了和基因的表达水平，并降低了和的表达。感染显著增加了桦树微克隆中、、、和转录本的水平；然而，在暴露于含有NPs的培养基并同时暴露于植物病原体时，、和基因的表达下降了5.4倍，这与NPs作用导致的致病负荷降低以及体外对克隆的同时刺激有关。所得结果可用于开发基于氧化铜纳米颗粒的制剂，用于树木作物克隆微繁殖过程中的消毒和刺激植物的植物免疫。
