Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Research Unit of Applied Microbiology and Pharmacology of Natural Substances, University of Abomey-Calavi, Godomey, Benin.
Sci Rep. 2022 Mar 10;12(1):4229. doi: 10.1038/s41598-022-08200-5.
Non-enterica subspecies of Salmonella enterica are rarely associated with human infections. Paradoxically, food safety legislations consider the entire genus Salmonella as pathogenic to humans. Globally, large amounts of seafoods are rejected and wasted due to findings of Salmonella. To inform better food safety decisions, we investigated the pathogenicity of Salmonella Salamae 42:r- and Salmonella Waycross isolated from Nile perch from Lake Victoria. Genome-wide analysis revealed absence of significant virulence determinants including on key Salmonella pathogenicity islands in both serovars. In epithelial cells, S. Salamae showed a weak invasion ability that was lower than the invH mutant of S. Typhimiurium used as negative control. Similarly, S. Salamae could not replicate inside macrophages. Moreover, intracellular replication in S. Waycross strains was significantly lower compared to the wild type S. Typhimurium. Our findings suggest a low pathogenicity of S. Salamae reinforcing the existing literature that non-enterica subspecies are avirulent. We propose that food legislations and actions taken on findings of Salmonella are revisited to avoid wasting valuable sea- and other foods.
沙门氏菌非肠亚种很少与人类感染有关。矛盾的是，食品安全法规将整个沙门氏菌属视为对人类具有致病性。在全球范围内，由于发现了沙门氏菌，大量的海鲜被拒绝和浪费。为了做出更好的食品安全决策，我们研究了从维多利亚湖尼罗河鲈鱼中分离出的沙门氏菌 Salamae 42:r-和沙门氏菌 Waycross 的致病性。全基因组分析显示，这两种血清型均缺乏包括关键沙门氏菌致病性岛在内的重要毒力决定因素。在肠上皮细胞中，沙门氏菌 Salamae 的侵袭能力较弱，低于用作阴性对照的沙门氏菌 Typhimurium invH 突变体。同样，沙门氏菌 Salamae 不能在巨噬细胞内复制。此外，与野生型沙门氏菌 Typhimurium 相比，沙门氏菌 Waycross 菌株的细胞内复制明显降低。我们的研究结果表明沙门氏菌 Salamae 的致病性较低，这加强了非肠亚种无毒力的现有文献。我们建议重新审视食品法规和对沙门氏菌检测结果采取的措施，以避免浪费宝贵的海鲜和其他食品。
