Bamunusinghage Nihal P D, Neelawala Roshen G, Magedara Hasintha P, Ekanayaka Nuwanthi W, Kalupahana Ruwani S, Silva-Fletcher Ayona, Kottawatta Sanda A
Wildlife Health Management Center, Department of Wildlife Conservation, Konduwatuwana, Ampara, 32000, Sri Lanka.
Department of Veterinary Public Health and Pharmacology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, 20400, Sri Lanka.
J Wildl Dis. 2022 Apr 1;58(2):380-383. doi: 10.7589/JWD-D-21-00048.
Antimicrobial resistance (AMR) is a major public health concern. We identified AMR in fecal Escherichia coli of wildlife (WL), urban wildlife (UWL), and livestock in the eastern region in Sri Lanka and compared the prevalence of AMR bacteria from carnivores, omnivores, and herbivores. Fecal samples were collected from 165 animals: WL (n=47), UWL (n=54), and livestock (n=64). Esherichia coli was cultured from 129 samples, with isolation rates of 76% from WL (36/47), 70% from UWL (45/54), and 75% from livestock (48/64). Testing E. coli isolates against 12 antimicrobials using the disk diffusion method revealed that the proportions of E. coli isolates resistant to at least one antimicrobial were WL 52.7%, UWL 20%, and livestock 52%. Multidrug-resistant E. coli isolates were detected in WL, UWL, and livestock. Overall, the prevalence of E. coli isolates with AMR was significantly lower in UWL compared with WL and livestock. The number of isolates showed AMR was significantly higher in E. coli from carnivores than in isolates from omnivores and in herbivores. We conclude that AMR E. coli in Sri Lanka is widespread in WL, UWL, and livestock. The higher incidence of AMR bacteria in carnivores compared with herbivores and omnivores suggest that the mechanisms of spread of AMR may vary among wild animals, which requires further investigation.
抗菌药物耐药性（AMR）是一个重大的公共卫生问题。我们在斯里兰卡东部地区的野生动物（WL）、城市野生动物（UWL）和家畜的粪便大肠杆菌中鉴定出了AMR，并比较了食肉动物、杂食动物和食草动物中AMR细菌的流行情况。从165只动物中采集了粪便样本：WL（n = 47）、UWL（n = 54）和家畜（n = 64）。从129个样本中培养出了大肠杆菌，分离率分别为：WL的76%（36/47）、UWL的70%（45/54）和家畜的75%（48/64）。使用纸片扩散法对大肠杆菌分离株进行12种抗菌药物检测，结果显示对至少一种抗菌药物耐药的大肠杆菌分离株比例分别为：WL 52.7%、UWL 20%和家畜52%。在WL、UWL和家畜中均检测到了多重耐药大肠杆菌分离株。总体而言，与WL和家畜相比，UWL中具有AMR的大肠杆菌分离株流行率显著较低。显示具有AMR的分离株数量在食肉动物的大肠杆菌中显著高于杂食动物和食草动物的分离株。我们得出结论，斯里兰卡的AMR大肠杆菌在WL、UWL和家畜中广泛存在。食肉动物中AMR细菌的发病率高于食草动物和杂食动物，这表明AMR的传播机制在野生动物中可能有所不同，这需要进一步调查。
