Department of Veterinary Public Health and Pharmacology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka.
Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Baru, Kelantan, Malaysia.
Zoonoses Public Health. 2024 Feb;71(1):84-97. doi: 10.1111/zph.13087. Epub 2023 Oct 25.
This study aimed to investigate and compare the proportion of AMR Escherichia coli (E. coli) between urban (Dompe in the Western province) and rural (Dambana in the Sabaragamuwa province) areas in Sri Lanka. The overall hypothesis of the study is that there is a difference in the proportion of AMR E. coli between the urban and the rural areas. Faecal samples were collected from healthy humans (n = 109), dairy animals (n = 103), poultry (n = 35), wild mammals (n = 81), wild birds (n = 76), soil (n = 80) and water (n = 80) from both areas. A total of 908 E. coli isolates were tested for susceptibility to 12 antimicrobials. Overall, E. coli isolated from urban area was significantly more likely to be resistant than those isolated from rural area. The human domain of the area had a significantly higher prevalence of AMR E. coli, but it was not significantly different in urban (98%) and rural (97%) areas. AMR E. coli isolated from dairy animals, wild animals and water was significantly higher in the urban area compared with the rural area. There was no significant difference in the proportion of multidrug resistance (MDR) E. coli isolated from humans, wild animals and water between the two study sites. Resistant isolates found from water and wild animals suggest contamination of the environment. A multi-sectorial One Health approach is urgently needed to control the spread of AMR and prevent the occurrences of AMR in Sri Lanka.
本研究旨在调查和比较斯里兰卡城乡（西部的 Dompe 和萨巴拉加穆瓦省的 Dambana）地区耐抗生素大肠杆菌（E. coli）的比例。该研究的总体假设是，城乡地区耐抗生素大肠杆菌的比例存在差异。从健康人类（n=109）、奶牛动物（n=103）、家禽（n=35）、野生动物（n=81）、野生鸟类（n=76）、土壤（n=80）和水（n=80）中收集粪便样本。共检测了 908 株 E. coli 对 12 种抗生素的敏感性。总体而言，从城市地区分离出的大肠杆菌比从农村地区分离出的大肠杆菌更有可能具有抗药性。该地区的人类领域具有更高的耐抗生素大肠杆菌流行率，但在城市（98%）和农村（97%）地区没有显著差异。与农村地区相比，城市地区从奶牛动物、野生动物和水中分离出的耐抗生素大肠杆菌明显更高。从人类、野生动物和水中分离出的多重耐药（MDR）大肠杆菌的比例在两个研究地点之间没有显著差异。从水和野生动物中发现的耐药菌表明环境受到了污染。迫切需要采取多部门的“同一健康”方法来控制抗生素耐药性的传播并防止抗生素耐药性在斯里兰卡的发生。
