Unidad Sureste, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Km 5.5 Carretera, Sierra Papacal-Chuburná, Chuburná, Mérida 97302, Yucatán, Mexico.
Unidad Noreste, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Autopista Mty-Aeropuerto, Vía de la Innovación 404, Parque PIIT, Cd Apodaca 66628, Nuevo León, Mexico.
Nutrients. 2022 Jan 18;14(3):422. doi: 10.3390/nu14030422.
Ionic calcium can help in the prevention of the process of osseous decalcification. This study aimed to evaluate the physicochemical properties and toxic effects of ionic calcium-fiber supplement () and its impact on bone health preservation in mice C57/BL6 fed a calcium-deficient diet. Physicochemical properties include FTIR, apparent calcium solubility estimated by the calcium ratio obtained by ionization chromatography and atomic absorption. In vitro genotoxicity and cytotoxicity of the a were assessed. Twenty-five 7-week-old C57/BL6 mice were fed calcium-free diet (CFD) or CFD plus CaCO (1.33 mg Ca) or CFD plus (1.33-6.66 mg Ca) for six weeks. After that, bone mass and microstructure parameters were assessed. Histological staining was performed to determine calcium deposits. (100%) exhibited an apparent calcium solubility higher than CaCO (12.3%). showed no cytotoxic and genotoxic in vitro activities. Histomorphometry analysis showed that the treated group displayed a higher trabecular number than the trabecular space. Also, the ratio BV/TV was increased compared with all treatments. Ionic calcium-fiber supplementation prevents bone deterioration compared to mice fed a calcium-deficient diet.
离子钙有助于防止骨质脱钙过程。本研究旨在评估离子钙-纤维补充剂（）的理化性质和毒副作用及其对喂食缺钙饮食的 C57/BL6 小鼠骨健康保护的影响。理化性质包括傅里叶变换红外光谱（FTIR）、通过离子色谱法获得的电离钙比估计的表观钙溶解度和原子吸收。评估了的体外遗传毒性和细胞毒性。将 25 只 7 周龄 C57/BL6 小鼠喂食无钙饮食（CFD）或 CFD 加 CaCO（1.33mgCa）或 CFD 加 （1.33-6.66mgCa）6 周。之后，评估骨量和微观结构参数。进行组织学染色以确定钙沉积物。（100%）表现出高于 CaCO（12.3%）的表观钙溶解度。在体外无细胞毒性和遗传毒性活性。组织形态计量学分析显示，与所有治疗组相比，经处理的组显示出更高的小梁数和更小的小梁间隙。此外，与所有处理相比，BV/TV 比值增加。与喂食缺钙饮食的小鼠相比，离子钙-纤维补充剂可预防骨恶化。
