Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Nutrients. 2022 Jan 30;14(3):608. doi: 10.3390/nu14030608.
Autism spectrum disorders (ASD) consist of a range of neurodevelopmental conditions accompanied by dysbiosis of gut microbiota. Therefore, a number of microbiota manipulation strategies were developed to restore their balance. However, a comprehensive comparison of the various methods on gut microbiota is still lacking. Here, we evaluated the effect of (BF) treatment and fecal microbiota transplantation (FT) on gut microbiota in a propionic acid (PPA) rat model of autism using 16S rRNA sequencing. Following PPA treatment, gut microbiota showed depletion of Bacteroidia and accompanied by a concomitant increase of , , and . The dysbiosis was predicted to cause increased levels of porphyrin metabolism and impairments of acyl-CoA thioesterase and ubiquinone biosynthesis. On the contrary, BF and FT treatments resulted in a distinct increase of , , , , and . The taxa in BF group positively correlated with vitamin B12 and flagella biosynthesis, while FT mainly enriched flagella biosynthesis. In contrast, BF and FT treatments negatively correlated with succinate biosynthesis, pyruvate metabolism, nitrogen metabolism, beta-Lactam resistance, and peptidoglycan biosynthesis. Therefore, the present study demonstrated that BF and FT treatments restored the PPA-induced dysbiosis in a treatment-specific manner.
自闭症谱系障碍（ASD）包括一系列神经发育障碍，伴有肠道微生物失调。因此，开发了许多微生物群操作策略来恢复其平衡。然而，各种方法对肠道微生物群的综合比较仍然缺乏。在这里，我们使用 16S rRNA 测序评估了双歧杆菌（BF）治疗和粪便微生物群移植（FT）对自闭症丙酸（PPA）大鼠模型肠道微生物群的影响。在 PPA 处理后，肠道微生物群显示出拟杆菌减少，同时伴随着梭菌增多，脱硫弧菌增多，和肠杆菌科增加。这种失调预计会导致卟啉代谢水平升高，并损害酰基辅酶 A 硫酯酶和泛醌生物合成。相反，BF 和 FT 处理导致特定的增加，例如双歧杆菌，普雷沃氏菌，罗氏菌，拟杆菌和消化链球菌。BF 组中的分类群与维生素 B12 和鞭毛生物合成呈正相关，而 FT 主要富集鞭毛生物合成。相反，BF 和 FT 处理与琥珀酸生物合成、丙酮酸代谢、氮代谢、β-内酰胺耐药和肽聚糖生物合成呈负相关。因此，本研究表明 BF 和 FT 治疗以特定的治疗方式恢复了 PPA 诱导的失调。
