School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Huizhou Health Sciences Polytechnic, Huizhou, China.
Pharm Biol. 2022 Dec;60(1):600-608. doi: 10.1080/13880209.2022.2047210.
(Blume & T. Nees) Torrend (Ganodermataceae) is an edible mushroom with medicinal properties. However, the effects of on gastric ulcer remain unclear.
To investigate the gastroprotective efficacy of water extract of (WEA) on gastric ulcer.
Sprague-Dawley rats were randomly grouped as control, model, lansoprazole and 200, 100 and 50 mg/kg of WEA. After pre-treatment for seven days, ethanol- and indomethacin-induced gastric ulcer models were established. The gastric ulcer and histopathology were investigated. Enzyme-linked immunosorbent assay (ELISA), quantitative polymerase chain reaction (Q-PCR) and Western blot assays were conducted to explore the potential anti-inflammatory effect and mechanism of WEA. Additionally, the pyloric ligation model was used to explore the influence of WEA on gastric acid and mucus.
Pre-treatment with WEA (200, 100 and 50 mg/kg) effectively reduced ulcerous area in both ethanol-induced (71%, 88% and 71%) and indomethacin-induced (77%, 65% and 86%) gastric ulcer model. The gastric levels of tumour necrosis factor-alpha (TNF-α) (34% and 50 mg/kg), interleukin-6 (IL-6) (32% and 100 mg/kg) and interleukin-1β (IL-1β) (36%, 45% and 41%) were reduced significantly ( < 0.05) by WEA. Serum nitric oxide was decreased significantly ( < 0.05) at 200 and 50 mg/kg and PGE2 concentration was increased remarkably ( < 0.05) at 100 mg/kg. Gene expression of inflammasome , and the nuclear translocation of nuclear factor-κB (NF-κB) P65 were significantly decreased by WEA pre-treatment. However, the pH of gastric acid and secretion of mucus did not show any significant change.
The gastroprotective effect of WEA on gastric damage is attributed to anti-inflammation through the inhibition on NF-κB P65 nuclear migration and gene expression.
（Blume & T. Nees）Torrend（灵芝科）是一种具有药用特性的可食用蘑菇。然而，关于其对胃溃疡的影响尚不清楚。
研究水提物（WEA）对胃溃疡的胃保护作用。
将 Sprague-Dawley 大鼠随机分为对照组、模型组、兰索拉唑组和 200、100 和 50mg/kg 的 WEA 组。预处理 7 天后，建立乙醇和吲哚美辛诱导的胃溃疡模型。研究胃溃疡和组织病理学。通过酶联免疫吸附试验（ELISA）、定量聚合酶链反应（Q-PCR）和 Western blot 分析探讨 WEA 的潜在抗炎作用和机制。此外，还使用幽门结扎模型探讨 WEA 对胃酸和黏液的影响。
WEA（200、100 和 50mg/kg）预处理可有效减少乙醇诱导（71%、88%和 71%）和吲哚美辛诱导（77%、65%和 86%）的胃溃疡模型中的溃疡面积。胃肿瘤坏死因子-α（TNF-α）（34%和 50mg/kg）、白细胞介素-6（IL-6）（32%和 100mg/kg）和白细胞介素-1β（IL-1β）（36%、45%和 41%）水平显著降低（ < 0.05）。血清一氧化氮在 200 和 50mg/kg 时显著降低（ < 0.05），前列腺素 E2 浓度在 100mg/kg 时显著升高（ < 0.05）。WEA 预处理可显著降低炎症小体基因表达和核因子-κB（NF-κB）P65 的核转位。然而，胃酸的 pH 值和黏液的分泌没有显示出任何显著变化。
WEA 对胃损伤的胃保护作用归因于通过抑制 NF-κB P65 核迁移和炎症小体基因表达来抗炎。
