沙棘多糖通过下调吸附、进入和氧化应激来抑制伪狂犬病病毒感染。

Hippophae rhamnoides polysaccharides dampen pseudorabies virus infection through downregulating adsorption, entry and oxidative stress.

作者信息

Huan Changchao, Xu Yao, Zhang Wei, Pan Haochun, Zhou Ziyan, Yao Jingting, Guo Tingting, Ni Bo, Gao Song

机构信息

Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China; Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, Jiangsu, China.

出版信息

Int J Biol Macromol. 2022 May 15;207:454-463. doi: 10.1016/j.ijbiomac.2022.03.041. Epub 2022 Mar 10.

DOI:10.1016/j.ijbiomac.2022.03.041

PMID:35278510

Abstract

Pseudorabies virus (PRV) is an alpha herpesvirus that causes pseudorabies. After 2011, new and more pathogenic PRV variants have caused huge economic losses to the pig industry. In addition, people have been reported to be infected with PRV. Therefore, developing new anti-PRV drugs is of great significance. In this study, we investigated the anti-PRV activity of Hippophae rhamnoides polysaccharides (HRP) in vitro. We found that HRP could significantly inhibit the infectivity of the PRV XJ5 strain in PK15 cells. Addition of HRP at different times of the virus life cycle mainly led to the inhibition of the adsorption and entry of virus into the cells. Our results revealed that HRP can reduce the malondialdehyde (MDA) content and reactive oxygen species (ROS) level in PRV-infected PK15 cells and increase the superoxide dismutase (SOD) activity. These results suggested that HRP can reduce PRV infection-induced oxidative stress. Therefore, HRP may act as an antiviral drug against newly emerging PRV variants.

摘要

伪狂犬病病毒（PRV）是一种引起伪狂犬病的α疱疹病毒。2011年以后，新的、致病性更强的PRV变异株给养猪业造成了巨大经济损失。此外，已有报道称人类感染了PRV。因此，开发新型抗PRV药物具有重要意义。在本研究中，我们在体外研究了沙棘多糖（HRP）的抗PRV活性。我们发现HRP能显著抑制PRV XJ5株在PK15细胞中的感染性。在病毒生命周期的不同时间添加HRP主要导致病毒吸附和进入细胞受到抑制。我们的结果表明，HRP可降低PRV感染的PK15细胞中丙二醛（MDA）含量和活性氧（ROS）水平，并提高超氧化物歧化酶（SOD）活性。这些结果提示，HRP可减轻PRV感染诱导的氧化应激。因此，HRP可能作为一种抗新型PRV变异株的抗病毒药物。