Huan Changchao, Zhou Ziyan, Yao Jingting, Ni Bo, Gao Song
Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
Molecules. 2022 Feb 13;27(4):1254. doi: 10.3390/molecules27041254.
Porcine pseudorabies (PR) is an important infectious disease caused by pseudorabies virus (PRV), which poses a major threat to food safety and security. Vaccine immunization has become the main means to prevent and control the disease. However, since 2011, a new PRV variant has caused huge economic losses to the Chinese pig industry. Panax notoginseng polysaccharides have immunomodulatory activity and other functions, but the antiviral effect has not been reported. We studied the anti-PRV activity of Panax notoginseng polysaccharides in vitro. A less cytopathic effect was observed by increasing the concentration of Panax notoginseng polysaccharides. Western blot, TCID, plaque assay, and IFA revealed that Panax notoginseng polysaccharides could significantly inhibit the infectivity of PRV XJ5 on PK15 cells. In addition, we also found that Panax notoginseng polysaccharides blocked the adsorption and replication of PRV to PK15 cells in a dose-dependent manner. These results show that Panax notoginseng polysaccharides play an antiviral effect mainly by inhibiting virus adsorption and replication in vitro. Therefore, Panax notoginseng polysaccharides may be a potential anti-PRV agent.
猪伪狂犬病（PR）是由伪狂犬病病毒（PRV）引起的一种重要传染病，对食品安全和保障构成重大威胁。疫苗免疫已成为预防和控制该疾病的主要手段。然而，自2011年以来，一种新的PRV变异株给中国养猪业造成了巨大经济损失。三七多糖具有免疫调节活性等功能，但其抗病毒作用尚未见报道。我们在体外研究了三七多糖的抗PRV活性。通过增加三七多糖浓度观察到细胞病变效应减轻。蛋白质免疫印迹法、半数组织培养感染剂量（TCID）、空斑试验和间接免疫荧光法（IFA）显示，三七多糖可显著抑制PRV XJ5对PK15细胞的感染性。此外，我们还发现三七多糖以剂量依赖方式阻断PRV对PK15细胞的吸附和复制。这些结果表明，三七多糖在体外主要通过抑制病毒吸附和复制发挥抗病毒作用。因此，三七多糖可能是一种潜在的抗PRV药物。
