地黄多糖对伪狂犬病毒具有抗病毒活性和抗氧化活性。

Rehmmannia glutinosa polysaccharide exerts antiviral activity against pseudorabies virus and antioxidant activity.

机构信息

Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China; Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou, China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China.

出版信息

Int J Biol Macromol. 2024 Aug;274(Pt 2):133455. doi: 10.1016/j.ijbiomac.2024.133455. Epub 2024 Jun 28.

DOI:10.1016/j.ijbiomac.2024.133455

PMID:38945342

Abstract

Pseudorabies virus (PRV) is an important pathogen harming the global pig industry. Vaccines available for swine cannot protect against PRV completely. Furthermore, no antiviral drugs are available to treat PRV infections. Rehmmannia glutinosa polysaccharide (RGP) possesses several medicinal properties. However, its antiviral activity is not reported. In the present study, we found that RGP can inhibit PRV/XJ5 infection by western blotting, immunofluorescent assay (IFA), and TCID assay quantitative polymerase chain reaction (qPCR). We revealed RGP can inhibit virus adsorption and invasion into PK-15 cells in a dose-dependent manner via western blotting, IFA, TCID assay, and quantitative polymerase chain reaction (qPCR), and suppressed PRV/XJ5 replication through western blotting, and qPCR. Additionally, it also reduced PRV/XJ5-induced ROS, lipid oxidation, and improved SOD levels in PK-15 cells, which was observed by using corresponding test kits. To conclude, our findings suggest that RGP might be a novel therapeutic agent for preventing and controlling PRV infection and antioxidant agent.

摘要

伪狂犬病病毒 (PRV) 是一种危害全球养猪业的重要病原体。现有的猪用疫苗不能完全预防 PRV，也没有抗病毒药物可用于治疗 PRV 感染。地黄多糖 (RGP) 具有多种药用特性。然而，其抗病毒活性尚未见报道。在本研究中，我们发现 RGP 可以通过 Western blot、免疫荧光检测（IFA）和 TCID 法 qPCR 抑制 PRV/XJ5 的感染。通过 Western blot、IFA、TCID 法 qPCR 证实，RGP 能以剂量依赖的方式抑制病毒吸附和侵入 PK-15 细胞，并通过 Western blot 和 qPCR 抑制 PRV/XJ5 的复制。此外，还通过相应的试剂盒观察到，它还能减少 PRV/XJ5 诱导的 PK-15 细胞中的 ROS、脂质氧化，并提高 SOD 水平。综上所述，我们的研究结果表明 RGP 可能是一种预防和控制 PRV 感染的新型治疗剂和抗氧化剂。