Ahmed Doaa M, Mohsen Abd El-Aziz M A, El-Deeb Mohamed A, Alkhedaide Adel, El-Tahan Amira M, Metwally El-Sayed M
Department of Plant Protection, Faculty of Agriculture, Zagazig University, Egypt.
Clinical Laboratory Sciences Department, Turabah University College, Taif University, 21995, Saudi Arabia.
Saudi J Biol Sci. 2022 Mar;29(3):1447-1455. doi: 10.1016/j.sjbs.2021.11.028. Epub 2021 Nov 24.
The present study aimed to evaluate the toxicity and biochemical changes of 3 instar larvae affected by neemazal T/S, clove oil and ginger oil. These compounds were evaluated compared to the recommended pesticide, Coragen 20% SC. by means of sublethal concentrations, LC and LC under constant laboratory conditions. Results showed that neemazal T/S is more toxic than detected oils compared with higher toxicity of coragen with LC values of 57.52, 159.94, 633.38 and 930.71 μg mL for coragen, neemazal, ginger oil and clove oil, respectively. There were highly significant differences between all treatments and untreated larvae. Neemazal possessed the greatest effect on activity level of most physiological parameters than selected oils. Larval content of digestive enzymes was decreased significantly 48 h after all treatments except for lipase, α-esterase and β-esterase (in case of coragen and clove oil). Also, total proteins, total carbohydrates, total lipids and total free amino acids take the same trend. Based on this study, these sublethal doses caused a significantly dose-dependent perturbation in determined components.
本研究旨在评估印楝素T/S、丁香油和姜油对3龄幼虫的毒性及生化变化。在恒定实验室条件下,通过亚致死浓度(LC)和LC,将这些化合物与推荐农药20%氯虫苯甲酰胺悬浮剂(Coragen 20% SC)进行比较评估。结果表明,与氯虫苯甲酰胺相比,印楝素T/S毒性更强,氯虫苯甲酰胺、印楝素、姜油和丁香油的LC值分别为57.52、159.94、633.38和930.71μg/mL。所有处理组与未处理幼虫之间存在极显著差异。印楝素对大多数生理参数活性水平的影响比所选油类更大。除脂肪酶、α-酯酶和β-酯酶(氯虫苯甲酰胺和丁香油处理情况)外,所有处理48小时后幼虫消化酶含量均显著降低。此外,总蛋白、总碳水化合物、总脂质和总游离氨基酸也呈现相同趋势。基于本研究,这些亚致死剂量在测定成分中引起了显著的剂量依赖性扰动。