Jalili Cyrus, Farzaei Mohammad Hossein, Rashidi Iraj, Mohammadnezamian Ayda, Ghanbari Ali
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Res Pharm Sci. 2022 Jan 15;17(2):209-218. doi: 10.4103/1735-5362.335178. eCollection 2022 Apr.
Dichlorvos, an organophosphate insecticide, induces side effects on normal tissues. On the other hand, Royal jelly (RJ) with antioxidant activities has many medical benefits including liver toxicity. In this study, we investigated the role of RJ in improving dichlorvos adverse impact on the liver of male rats.
Forty-eight male rats were randomly divided into 8 groups (n = 6); receiving by gavage normal saline (0.09%), dichlorvos (4 mg/kg/day), RJ (50, 100, 150 mg/kg/day; RJ 1, 2, 3) or dichlorvos + RJs, daily for 28 consecutive days. At the end of experiments, histopathology alterations, apoptosis induction, and biochemical factors related to the liver were evaluated.
FINDINGS/RESULTS: There was a significant reduction in the number of hepatocytes and total antioxidant capacity (TAC) levels in the dichlorvos group compared to the control group, whereas these parameters in the dichlorvos + RJs groups, were significantly increased compared to the dichlorvos group. Central vein diameter, liver enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) serum levels of nitric oxide, and apoptotic index were significantly higher in the dichlorvos group than in the control, while these parameters were decreased in the dichlorvos + RJs groups versus the dichlorvos group.
RJ at 50 mg/kg protected dichlorvos-induced liver damage in rats. Dichlorvos- hepatitis mechanism could be oxidative induction as long as antioxidant reduction leads to apoptosis in this organ, while RJ due to its antioxidant potential suppresses this hazardous cellular and molecular process.
敌敌畏是一种有机磷杀虫剂，会对正常组织产生副作用。另一方面，具有抗氧化活性的蜂王浆（RJ）有诸多医学益处，包括对肝脏毒性的作用。在本研究中，我们探究了蜂王浆在改善敌敌畏对雄性大鼠肝脏不良影响方面的作用。
48只雄性大鼠随机分为8组（每组n = 6）；连续28天每天经口灌胃给予生理盐水（0.09%）、敌敌畏（4毫克/千克/天）、蜂王浆（50、100、150毫克/千克/天；分别为RJ 1、RJ 2、RJ 3）或敌敌畏 + 蜂王浆。实验结束时，评估肝脏的组织病理学改变、凋亡诱导情况以及与肝脏相关的生化指标。
与对照组相比，敌敌畏组的肝细胞数量和总抗氧化能力（TAC）水平显著降低，而与敌敌畏组相比，敌敌畏 + 蜂王浆组的这些参数显著升高。敌敌畏组的中央静脉直径、肝酶（天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶）、血清一氧化氮水平及凋亡指数均显著高于对照组，而与敌敌畏组相比，敌敌畏 + 蜂王浆组的这些参数有所降低。
50毫克/千克的蜂王浆可保护大鼠免受敌敌畏诱导的肝脏损伤。敌敌畏性肝炎的机制可能是氧化诱导，因为抗氧化能力降低会导致该器官细胞凋亡，而蜂王浆因其抗氧化潜力可抑制这种有害的细胞和分子过程。
