Ghobadi Sahel, Dastan Dara, Soleimani Meysam, Nili-Ahmadabadi Amir
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, I.R. Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, I.R. Iran.
Res Pharm Sci. 2019 Dec 11;14(6):488-495. doi: 10.4103/1735-5362.272535. eCollection 2019 Dec.
() is a species of wild native to northwest Iran that its hepatoprotective effects have not yet been confirmed. This study investigated the effect of plant against acetaminophen (APAP)-induced acute liver damage. After preliminary studies, the methanol fraction (ATMF) was selected for study. Thirty-six rats were divided into six groups of 6 each and treated by gavage as follows: groups 1 and 2 received normal saline; group 3 received 400 mg/kg of ATMF; and groups 4-6 were treated with 100, 200, and 400 mg/kg of ATMF, respectively. After two consecutive weeks, except groups 1 and 3, rats were administered with an oral single dose of APAP (2 g/kg). After 48 h, blood and liver samples were collected for histological and biochemical examinations. The results showed that APAP caused a significant increase in alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and alkaline phosphatase serum levels, lipid peroxidation (all with < 0.001) and hepatic nitric oxide ( < 0.01). In addition, APAP led to the depletion of the total antioxidant capacity, total thiol group (both with < 0.001), and structural alterations in the hepatic tissue. Following administration of ATMF extract, a significant improvement was observed in the functional and oxidative stress markers of hepatic tissue alongside histopathologic changes. In conclusion, the present study showed that the administration of ATMF might prevent hepatic oxidative damage by improving oxidant/antioxidant balance in animals exposed to APAP.
（）是一种原产于伊朗西北部的野生植物，其肝脏保护作用尚未得到证实。本研究调查了该植物对乙酰氨基酚（APAP）诱导的急性肝损伤的影响。经过初步研究，选择了该植物的甲醇提取物（ATMF）进行研究。将36只大鼠分为6组，每组6只，通过灌胃进行如下处理：第1组和第2组给予生理盐水；第3组给予400mg/kg的ATMF；第4 - 6组分别用100、200和400mg/kg的ATMF处理。连续两周后，除第1组和第3组外，给大鼠口服单剂量的APAP（2g/kg）。48小时后，采集血液和肝脏样本进行组织学和生化检查。结果表明，APAP导致丙氨酸转氨酶、天冬氨酸转氨酶、乳酸脱氢酶和碱性磷酸酶血清水平显著升高，脂质过氧化（均P<0.001）和肝脏一氧化氮水平升高（P<0.01）。此外，APAP导致总抗氧化能力、总巯基含量降低（均P<0.001），并使肝组织发生结构改变。给予ATMF提取物后，肝组织的功能和氧化应激标志物以及组织病理学变化均有显著改善。总之，本研究表明，给予ATMF可能通过改善暴露于APAP的动物体内的氧化/抗氧化平衡来预防肝脏氧化损伤。
