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蜂王浆对链脲佐菌素诱导的糖尿病大鼠肝脏和胰腺血清生化改变及氧化应激的改善作用

Improvement in Serum Biochemical Alterations and Oxidative Stress of Liver and Pancreas following Use of Royal Jelly in Streptozotocin-Induced Diabetic Rats.

作者信息

Ghanbari Elham, Nejati Vahid, Khazaei Mozafar

机构信息

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.

出版信息

Cell J. 2016 Fall;18(3):362-70. doi: 10.22074/cellj.2016.4564. Epub 2016 Aug 24.

Abstract

OBJECTIVE

This study aimed to evaluate the effects of royal jelly (RJ) on serum biochemical alterations and oxidative stress status in liver and pancreas of streptozotocin (STZ)- induced diabetic rats.

MATERIALS AND METHODS

In this experimental study, thirty two male Wistar rats were divided into the following four groups (n=8/group): i. Control (C), ii. Diabetic (D), iii. Royal jelly (R), and iv. Royal jelly-treated diabetic (D/R) groups. Diabetes was induced by single intraperitoneal (IP) injection of STZ (60 mg/kg). The RJ [100 mg/kg body weight (BW)] was administered orally for 42 days. Blood samples were used to determine serum levels of insulin, high density lipoprotein cholesterol (HDL-c), total protein (TP), albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and fasting blood glucose (FBG). Also, the antioxidant status was evaluated by determining the levels of malondialdehyde (MDA), catalase (CAT) and ferric reducing antioxidant power (FRAP) in liver and pancreas. Data were analyzed by one-way analysis of variance (ANOVA) with P<0.05 as the significant level.

RESULTS

STZ-induced diabetic rats showed a significant elevation in the serum levels of AST, ALT, ALP and FBG, whereas there was a significant decrease in serum levels of insulin, albumin, HDL-c and TP (P<0.05). Treatment of the diabetic rats with RJ restored the changes of the above parameters to their normal levels (P<0.05). In addition, RJ significantly improved reduced levels of FRAP and CAT as well as high MDA level in liver and pancreas (P<0.05).

CONCLUSION

RJ improves oxidative damage induced by STZ in the liver and pancreas of rats; therefore, it can be considered as an effective and alternative treatment for diabetes.

摘要

目的

本研究旨在评估蜂王浆(RJ)对链脲佐菌素(STZ)诱导的糖尿病大鼠肝脏和胰腺血清生化改变及氧化应激状态的影响。

材料与方法

在本实验研究中,32只雄性Wistar大鼠被分为以下四组(每组n = 8):i. 对照组(C),ii. 糖尿病组(D),iii. 蜂王浆组(R),iv. 蜂王浆治疗糖尿病组(D/R)。通过单次腹腔注射STZ(60 mg/kg)诱导糖尿病。以100 mg/kg体重(BW)口服给予RJ,持续42天。采集血样以测定血清胰岛素、高密度脂蛋白胆固醇(HDL-c)、总蛋白(TP)、白蛋白、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和空腹血糖(FBG)水平。此外,通过测定肝脏和胰腺中丙二醛(MDA)、过氧化氢酶(CAT)和铁还原抗氧化能力(FRAP)水平来评估抗氧化状态。数据采用单因素方差分析(ANOVA)进行分析,以P < 0.05为显著水平。

结果

STZ诱导的糖尿病大鼠血清AST、ALT、ALP和FBG水平显著升高,而血清胰岛素、白蛋白、HDL-c和TP水平显著降低(P < 0.05)。用RJ治疗糖尿病大鼠可使上述参数变化恢复至正常水平(P < 0.05)。此外,RJ显著改善了肝脏和胰腺中降低的FRAP和CAT水平以及升高的MDA水平(P < 0.05)。

结论

RJ可改善STZ诱导的大鼠肝脏和胰腺氧化损伤;因此,它可被视为糖尿病的一种有效替代治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b0/5011324/288fa1a8d488/Cell-J-18-362-g01.jpg

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