狂犬病病毒多克隆和单克隆抗体偶联物产生的免疫荧光反应的稳定性。
Stability of immunofluorescence reactions produced by polyclonal and monoclonal antibody conjugates for rabies virus.
作者信息
Durham T M, Smith J S, Reid F L, Hale-Smith C T, Fears M B
出版信息
J Clin Microbiol. 1986 Aug;24(2):301-3. doi: 10.1128/jcm.24.2.301-303.1986.
We investigated two consumer complaints that described fading of immunofluorescence reactions associated with the use of a commercial antirabies, fluorescein-labeled, monoclonal antibody conjugate. We compared the performance of this product with that of two polyclonal antibody antirabies conjugates and observed significant diminution of fluorescence with the monoclonal antibody conjugate only. Furthermore, the fading occurred only on tissue impressions that had been mounted but not exposed to UV light excitation, thereby essentially eliminating the photobleaching associated with fluorescence microscopy as a causative factor. Our observations suggest that mounting medium pH and the holding temperature of stained slides may be critical factors in maintaining optimal immunofluorescence reactions with this monoclonal antibody conjugate. We discuss some probable mechanisms that could produce the type of fading observed and also suggest certain precautionary measures for use with this monoclonal antibody conjugate.
我们调查了两起消费者投诉，投诉描述了与使用一种商业化的抗狂犬病荧光素标记单克隆抗体偶联物相关的免疫荧光反应褪色情况。我们将该产品的性能与两种多克隆抗体抗狂犬病偶联物的性能进行了比较，结果发现只有单克隆抗体偶联物出现了明显的荧光减弱。此外，褪色仅发生在已封固但未暴露于紫外线激发的组织印片上，从而基本排除了与荧光显微镜相关的光漂白作为一个致病因素。我们的观察结果表明，封固介质的pH值和染色玻片的保存温度可能是维持与这种单克隆抗体偶联物最佳免疫荧光反应的关键因素。我们讨论了一些可能导致观察到的褪色类型的机制，并还提出了使用这种单克隆抗体偶联物的某些预防措施。
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