Gu Xinyi, Zhang Shuyi, Ma Weini, Wang Qixue, Li Ying, Xia Chenyi, Xu Ying, Zhang Ting, Yang Li, Zhou Mingmei
Institute for Interdisciplinary Medicine Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Shanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Front Microbiol. 2022 Feb 25;13:778512. doi: 10.3389/fmicb.2022.778512. eCollection 2022.
Based on our previous research, chronic paradoxical sleep deprivation (PSD) can cause depression-like behaviors and microbial changes in gut microbiota. Coffee, as the world's most popular drink for the lack of sleep, is beneficial to health and attention and can eliminate the cognitive sequelae caused by poor sleep. The purpose of this study is to investigate the effects of coffee and decaffeinated coffee on PSD rats.
A total of 32 rats were divided into four groups: control group, PSD model group, conventional coffee group, and decaffeinated coffee group. Behavioral tests, including sucrose preference test, open field test, forced swimming test, and tail suspension test, as well as biochemical detection for inflammatory and antioxidant indexes were performed. The effects of coffee and decaffeinated coffee on the gut microbiota of PSD rats were investigated by 16S rRNA gene sequencing.
Coffee and decaffeinated coffee significantly improved the depression-like behaviors. Moreover, the serum levels of interleukin-6 and tumor necrosis factor alpha were decreased in both coffee and decaffeinated coffee groups, as well as the levels of superoxide dismutase and GSH-Px were increased. Gut microbiota analysis revealed that the abundance of , , , and were significantly increased in PSD rats, while the abundance of and were significantly decreased. After the treatment of coffee and decaffeinated coffee, the abundance of the above gut microbiota was all restored in different degrees. Coffee had relatively more significant effects on PSD-induced depressive-like behaviors, while the difference between coffee and decaffeinated coffee was not obvious in correcting the disorder of gut microbiota.
These findings have shown that both coffee and decaffeinated coffee are effective for sleep deprivation-induced depression-like behaviors and the dysbiosis of gut microbiota and indicated that caffeine may be not the only key substance of coffee for regulating gut microbiota.
基于我们之前的研究，慢性矛盾性睡眠剥夺（PSD）可导致抑郁样行为和肠道微生物群的变化。咖啡作为世界上最受欢迎的助眠饮品，对健康和注意力有益，并且可以消除睡眠不足引起的认知后遗症。本研究的目的是探讨咖啡和脱咖啡因咖啡对PSD大鼠的影响。
将32只大鼠分为四组：对照组、PSD模型组、常规咖啡组和脱咖啡因咖啡组。进行行为测试，包括蔗糖偏好测试、旷场试验、强迫游泳试验和悬尾试验，以及炎症和抗氧化指标的生化检测。通过16S rRNA基因测序研究咖啡和脱咖啡因咖啡对PSD大鼠肠道微生物群的影响。
咖啡和脱咖啡因咖啡均显著改善了抑郁样行为。此外，咖啡组和脱咖啡因咖啡组的血清白细胞介素-6和肿瘤坏死因子α水平均降低，超氧化物歧化酶和谷胱甘肽过氧化物酶水平均升高。肠道微生物群分析显示，PSD大鼠中、、和的丰度显著增加，而和的丰度显著降低。经咖啡和脱咖啡因咖啡处理后，上述肠道微生物群的丰度均有不同程度的恢复。咖啡对PSD诱导的抑郁样行为影响相对更显著，而咖啡和脱咖啡因咖啡在纠正肠道微生物群紊乱方面差异不明显。
这些研究结果表明，咖啡和脱咖啡因咖啡对睡眠剥夺诱导的抑郁样行为和肠道微生物群失调均有效，并表明咖啡因可能不是咖啡调节肠道微生物群的唯一关键物质。
