Velders Aldrik H, Ossendrijver Michel, Keijser Bart J F, Saggiomo Vittorio
Laboratory of BioNanoTechnology Wageningen University P.O. Box 8038 Wageningen 6700EK The Netherlands.
TNO Microbiology and Systems Biology P.O. Box 360 Zeist 3700 AJ The Netherlands.
Glob Chall. 2021 Dec 26;6(3):2100078. doi: 10.1002/gch2.202100078. eCollection 2022 Mar.
During the SARS-CoV2 pandemic, it has become clear that centralized testing suffers from multiple bottlenecks. Logistics, number of machines, and people available to run the diagnostic tests are limited. A solution to those bottlenecks would be a fully decentralized system, where people can test themselves at home and only report back the outcome of the test in a centralized database. Here a noninstrumental device capable of achieving isothermal conditions useful for detecting the SARS-CoV2 RNA using loop mediated amplification (LAMP) tests is presented. This device, compared to others reported in literature or present on the market, is cheap, easy to produce and use, and has little impact on the environment. Using a simple aluminum coffee capsule, a phase change material, and a 3D printed holder, this device, when placed in boiling water, is able to maintain a temperature of 65 °C for 25 min, required for running the LAMP reaction. In principle, this device can be applied to any LAMP reaction, and hence employed for many different applications, and can be deployed in large quantities in short amount of time.
在新冠疫情期间，很明显集中检测存在多个瓶颈。物流、用于进行诊断测试的机器数量和人员都有限。解决这些瓶颈的一个办法是建立一个完全分散的系统，人们可以在家中自行检测，然后只将检测结果上报到一个集中式数据库。本文介绍了一种非仪器设备，它能够实现等温条件，利用环介导等温扩增（LAMP）测试来检测新冠病毒RNA。与文献中报道的或市场上现有的其他设备相比，该设备价格便宜、易于生产和使用，并且对环境影响很小。通过使用一个简单的铝制咖啡胶囊、一种相变材料和一个3D打印的支架，该设备在放入沸水中时，能够将温度维持在65°C达25分钟，这是进行LAMP反应所需的时间。原则上，该设备可应用于任何LAMP反应，因此可用于许多不同的应用，并且能够在短时间内大量部署。
