Zherebtsov Evgenii A, Potapova Elena V, Mamoshin Andrian V, Shupletsov Valery V, Kandurova Ksenia Y, Dremin Viktor V, Abramov Andrey Y, Dunaev Andrey V
Research & Development Center of Biomedical Photonics, Orel State University, Orel, Russia.
Optoelectronics and Measurement Techniques unit, University of Oulu, Oulu, Finland.
Biomed Opt Express. 2022 Jan 6;13(2):633-646. doi: 10.1364/BOE.447687. eCollection 2022 Feb 1.
This work presents results of in vivo and in situ measurements of hepatocellular carcinoma by a developed optical biopsy system. Here, we describe the technical details of the implementation of fluorescence lifetime and diffuse reflectance measurements by the system, equipped with an original needle optical probe, compatible with the 17.5G biopsy needle standard. The fluorescence lifetime measurements observed by the setup were verified in fresh solutions of NADH and FAD, and then applied in a murine model for the characterisation of inoculated hepatocellular carcinoma (HCC) and adjacent liver tissue. The technique, applied in vivo and in situ and supplemented by measurements of blood oxygen saturation, made it possible to reveal statistically significant transformation in the set of measured parameters linked with the cellular pools of NADH and NADPH. In the animal model, we demonstrate that the characteristic changes in registered fluorescent parameters can be used to reliably distinguish the HCC tissue, liver tissue in the control, and the metabolically changed liver tissues of animals with the developed HCC tumour. For further transition to clinical applications, the optical biopsy system was tested during the routing procedure of the PNB in humans with suspected HCC. The comparison of the data from murine and human HCC tissues suggests that the tested animal model is generally representative in the sense of the registered fluorescence lifetime parameters, while statistically significant differences between their absolute values can still be observed.
这项工作展示了通过一种研发的光学活检系统对肝细胞癌进行体内和原位测量的结果。在此，我们描述了该系统配备与17.5G活检针标准兼容的原创针状光学探头实施荧光寿命和漫反射测量的技术细节。该装置所观察到的荧光寿命测量结果在NADH和FAD的新鲜溶液中得到验证，然后应用于小鼠模型，以表征接种的肝细胞癌（HCC）及相邻肝组织。该技术在体内和原位应用，并辅以血氧饱和度测量，使得揭示与NADH和NADPH细胞池相关的一组测量参数中的统计学显著变化成为可能。在动物模型中，我们证明所记录的荧光参数的特征变化可用于可靠地区分HCC组织、对照中的肝组织以及患有已发展HCC肿瘤的动物的代谢改变肝组织。为了进一步向临床应用过渡，在对疑似HCC的人类进行PNB的常规程序中对光学活检系统进行了测试。来自小鼠和人类HCC组织的数据比较表明，就所记录的荧光寿命参数而言，所测试的动物模型总体上具有代表性，但其绝对值之间仍可观察到统计学显著差异。
