Velázquez-Martínez Victor, Valles-Rosales Delia, Rodríguez-Uribe Laura, Laguna-Camacho Juan Rodrigo, López-Calderón Hector Daniel, Delgado Efren
Industrial Engineering, New Mexico State University, Las Cruces, NM, United States.
Department of Family and Consumer Sciences, New Mexico State University, Las Cruces, NM, United States.
Front Nutr. 2022 Feb 24;9:834557. doi: 10.3389/fnut.2022.834557. eCollection 2022.
Several parameters, including particle size, solvent, temperature, and extraction method, affect phenolic compounds' extraction yield from a plant matrix. Considering the wide availability of sugarcane bagasse (SCB), this study analyzed the effect of different extraction methods and geographical origins on the yield, quality, and antimicrobial activity of phenolic compounds from SCB extracts. Samples from three geographical locations (Veracruz, Mexico; Santa Rosa, Texas, USA; and St. Mary, Louisiana, USA) were analyzed. Extraction was performed using an orbital shaker or ultrasonic bath at various times at a fixed temperature of 50°C, with 90% ethanol or methanol. The highest yield (5.91 mg GAE) was obtained using an orbital shaker for 24 h with 90% methanol as the solvent. HPLC-MS identified desferrioxamine b, baicalein, madecassic acid, and podototarin at different concentrations in all three SCB samples. The antimicrobial activity of these compounds was tested against , and . The antimicrobial activity was also tested against modifications of the : the MutL Homolog 1 (MLH1), Slow Growth Suppressor (), O-6-MethylGuanine-DNA methyltransferase (), and RADiation sensitive (), carrying mutations related to different cancer types. In addition, the results were compared with the effect of ampicillin and kanamycin. The SCB extracts showed up to 90% growth inhibition against at 200-800 μg/mL and 50% growth inhibition against at 800 μg/mL. The inhibitory effect against modified yeast , RAD, and was 50-80% at 800 μg/mL. The percentage of inhibition and the phenolic compound contents differed depending on the origin of the SCB sample. These findings are promising for using this industrial byproduct to obtain compounds for nutraceutical, food additive, or medical uses.
包括粒径、溶剂、温度和提取方法在内的几个参数会影响从植物基质中提取酚类化合物的产率。考虑到甘蔗渣（SCB）的广泛可得性，本研究分析了不同提取方法和地理来源对SCB提取物中酚类化合物的产率、质量和抗菌活性的影响。分析了来自三个地理位置（墨西哥韦拉克鲁斯；美国得克萨斯州圣罗莎；美国路易斯安那州圣玛丽）的样本。在50°C的固定温度下，使用轨道振荡器或超声浴，以90%乙醇或甲醇为溶剂，在不同时间进行提取。使用轨道振荡器，以90%甲醇为溶剂提取24小时，获得了最高产率（5.91毫克没食子酸当量）。HPLC-MS在所有三个SCB样品中鉴定出不同浓度的去铁胺b、黄芩苷、羟基积雪草苷和足叶草毒素。测试了这些化合物对[具体菌种1]、[具体菌种2]和[具体菌种3]的抗菌活性。还测试了这些化合物对[具体酵母菌株]的变体的抗菌活性：MutL同源物1（MLH1）、生长缓慢抑制因子（[具体名称1]）、O-6-甲基鸟嘌呤-DNA甲基转移酶（[具体名称2]）和辐射敏感（[具体名称3]），它们携带与不同癌症类型相关的突变。此外，将结果与氨苄青霉素和卡那霉素的效果进行了比较。SCB提取物在200-800μg/mL时对[具体菌种1]的生长抑制率高达90%，在800μg/mL时对[具体菌种2]的生长抑制率为50%。在800μg/mL时，对改良酵母[具体酵母菌株]、RAD和[具体名称3]的抑制效果为50-80%。抑制百分比和酚类化合物含量因SCB样品的来源而异。这些发现对于利用这种工业副产品获得用于营养保健品、食品添加剂或医疗用途的化合物很有前景。
