Liao Cong, Wang Tao, Koehler Anson V, Hu Min, Gasser Robin B
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia.
Curr Res Parasitol Vector Borne Dis. 2021 Apr 24;1:100025. doi: 10.1016/j.crpvbd.2021.100025. eCollection 2021.
is a group of protistan parasites of a range of vertebrates including mammals and birds. Stimulated by previous work that revealed "zoonotic" subtypes (i.e. IIIbA26G1R1b and IIIbA22G1R1c) in diarrhoeic children and domestic chickens in Wuhan city and environs in Hubei Province, China, here we explored whether zoonotic subtypes might also occur in pet birds in Wuhan city. From 11 bird markets in this city, we collected 322 faecal samples from 48 species of birds (representing six taxonomic orders), isolated genomic DNA and then conducted PCR-based sequencing of genetic markers in the small subunit () of the nuclear ribosomal RNA and the 60 kDa glycoprotein () genes of . Using , was detected in 55 (17%) of the 322 samples. , , , and were characterised in 18%, 47%, 11%, 2% and 20% of the 55 samples, respectively, and a novel -like taxon in one sample Using , only one subtype (IIIeA17G2R1) of was identified, which had not been detected in a previous study of diarrhoeic children in Wuhan. However, IIIe subtypes have been found in both humans and birds around the world. The relatively high prevalence and genetic diversity of recorded here in pet birds raise awareness about possible reservoirs of zoonotic variants of in birds in Wuhan, and potentially, other provinces in China.
是一组寄生于包括哺乳动物和鸟类在内的多种脊椎动物的原生动物寄生虫。受先前研究的启发，该研究揭示了在中国湖北省武汉市及周边地区腹泻儿童和家鸡中存在“人畜共患”亚型（即IIIbA26G1R1b和IIIbA22G1R1c），我们在此探讨了人畜共患亚型是否也可能出现在武汉市的宠物鸟中。我们从该市的11个鸟类市场收集了来自48种鸟类（代表六个分类目）的322份粪便样本，分离出基因组DNA，然后对核糖体RNA小亚基（）和的60 kDa糖蛋白（）基因中的遗传标记进行基于PCR的测序。使用，在322份样本中的55份（17%）中检测到了。在55份样本中，分别有18%、47%、11%、2%和20%的样本鉴定出了、、、和，并且在一个样本中鉴定出了一个新的类taxon。使用，仅鉴定出了的一种亚型（IIIeA17G2R1），该亚型在先前对武汉腹泻儿童的研究中未被检测到。然而，IIIe亚型在世界各地的人类和鸟类中均有发现。此处记录的宠物鸟中相对较高的流行率和遗传多样性提高了人们对武汉鸟类中可能存在的人畜共患变体储存库以及中国其他省份潜在储存库的认识。
