Wu Yang, Li Zhiying, Shen Youming
Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.
Key Laboratory of National Forestry & Grassland Bureau for Plant Fiber Functional Materials, Fujian Agriculture and Forestry University, Fuzhou 350108, P. R. China.
ACS Omega. 2019 Sep 19;4(14):16242-16246. doi: 10.1021/acsomega.9b02594. eCollection 2019 Oct 1.
Hydrogen peroxide (HO) is a majority reactive oxygen species (ROS) and acts as an essential role in pathological and physiological processes. Therefore, the development of quantitative detection of methods for HO is necessary. Here, we constructed of a novel simple fluorescence probe for detection of HO based on the excited-state intramolecular proton transfer process. The probe utilized a phthalimide derivative as the fluorophore and selected phenylboronic acid as the recognition site for HO. In response to HO, the probe exhibited 63-fold fluorescence intensity enhancement, a low detection limit (8.4 × 10 M), and large Stokes shift (111 nm). In addition, the probe displayed high selectivity for HO over other ROS. Moreover, the probe was successfully employed for imaging of HO in living cells.
过氧化氢（HO）是一种主要的活性氧物种（ROS），在病理和生理过程中起着重要作用。因此，开发用于HO的定量检测方法是必要的。在此，我们基于激发态分子内质子转移过程构建了一种新型的简单荧光探针用于检测HO。该探针利用邻苯二甲酰亚胺衍生物作为荧光团，并选择苯硼酸作为HO的识别位点。在与HO反应时，该探针表现出63倍的荧光强度增强、低检测限（8.4×10⁻⁸ M）和大斯托克斯位移（111 nm）。此外，该探针对HO表现出高于其他ROS的高选择性。而且，该探针已成功用于活细胞中HO的成像。
