Paje Leo Adrianne, Choi Jungwon, Lee Hak-Dong, Kim Juree, Yu A Ram, Bae Min-Jung, Geraldino Paul John L, Lee Sanghyun
Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Technical Assistance Department, The Food Industry Promotional Agency of Korea, Iksan 54576, Republic of Korea.
Heliyon. 2022 Mar 4;8(3):e09046. doi: 10.1016/j.heliyon.2022.e09046. eCollection 2022 Mar.
We isolated and purified phenolic acids and flavonoids from the ethanolic extract of using silica gel and a Sephadex LH-20 column chromatography. Spectroscopy revealed the isolated compounds were caffeic acid, rosmarinic acid, hispidulin, luteolin, jaceosidin, nepitrin, homoplantaginin, 6-hydroxyluteolin 7--glucoside, 6-methoxynaringenin 7--glucoside, naasanone, and cosmosiin. Quantitative analyses, using high-performance liquid chromatography coupled with UV (HPLC-UV), revealed that the major flavonoid from was 6-hydroxyluteolin 7--glucoside (100.63 mg/g) and the most abundant phenolic acid was rosmarinic acid (47.73 mg/g). Furthermore, among four other species, contained the highest overall phenolic acid and flavonoid level but these were still lower than . These results can help assess the potential of phenolic acids and flavonoids as potent sources of pharmacological ingredients from different species extracts.
我们使用硅胶和葡聚糖凝胶LH - 20柱色谱法从[植物名称]的乙醇提取物中分离并纯化了酚酸和黄酮类化合物。光谱分析表明，分离出的化合物为咖啡酸、迷迭香酸、毛地黄黄酮、木犀草素、雅可西定、尼泊尔黄酮素、高车前苷、6 - 羟基木犀草素7 - O - 葡萄糖苷、6 - 甲氧基柚皮素7 - O - 葡萄糖苷、奈三酮和紫花前胡苷。采用高效液相色谱 - 紫外联用技术（HPLC - UV）进行定量分析，结果显示[植物名称]中主要的黄酮类化合物为6 - 羟基木犀草素7 - O - 葡萄糖苷（100.63 mg/g），含量最丰富的酚酸为迷迭香酸（47.73 mg/g）。此外，在其他四种[植物名称]中，[植物名称]的酚酸和黄酮类化合物总量最高，但仍低于[另一种植物名称]。这些结果有助于评估酚酸和黄酮类化合物作为不同[植物名称]提取物中潜在药理成分来源的潜力。
