Moshari-Nasirkandi Raheleh, Alirezalu Abolfazl, Chamanabad Hamid Reza Mohammaddoust, Amato Jussara, Alipour Hadi, Asghari Ali, Moshari-Nasirkandi Atefeh
Department of Plant production and genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran.
Sci Rep. 2024 Dec 30;14(1):32064. doi: 10.1038/s41598-024-83756-y.
In this study, screening of the collected 70 Salvia nemorosa L. populations from 54 habitats from West Azerbaijan province, Iran was evaluated by analyzing the content of phytochemical compounds, antioxidant activity, and UHPLC-HRMS profiling in different populations. The aerial parts of the plants were analyzed based on total phenolic (TPC) and flavonoid (TFC), total tannin (TTC), ascorbic acid (AAC), chlorophylls (Cla, and Clb), total carotenoid (TCC), β-carotene, antioxidant activity (by DPPH and FRAP assays), and 40 polyphenolic compounds by UHPLC-HRMS (phenolic acids, flavonoids and fatty acyl glicosides). Significant variations in phytochemical compositions and antioxidant activity were observed among S. nemorosa samples from different habitats. Rosmarinic acid, sagerinic acid, and caffeoylquinic acids (notable polyphenolic compounds with significant pharmacological properties, particularly in the context of their antioxidant, anti-inflammatory, and enzyme inhibitory activities) were identified as the major compounds in S. nemorosa populations. According to hierarchical cluster analysis (HCA) three groups of species were identified based on phytochemical compounds and antioxidant activity. Data analysis indicated that the quality and quantity of the chemical compounds, as well as their antioxidant functions, varied depending on habitat diversity. Overall, the results showed that each population exhibited a unique class of polyphenols, highlighting diverse potential applications across various industries. These findings suggest that the S. nemorosa populations studied here have significant potential for the development of innovative nutraceuticals, addressing the current scarcity of S. nemorosa-derived products in the market.
在本研究中，通过分析不同种群中植物化学成分的含量、抗氧化活性和超高效液相色谱-高分辨质谱（UHPLC-HRMS）图谱，对从伊朗西阿塞拜疆省54个栖息地采集的70个森林鼠尾草种群进行了筛选。基于总酚（TPC）、黄酮（TFC）、总单宁（TTC）、抗坏血酸（AAC）、叶绿素（叶绿素a和叶绿素b）、总类胡萝卜素（TCC）、β-胡萝卜素、抗氧化活性（通过DPPH和FRAP测定）以及通过UHPLC-HRMS分析40种多酚化合物（酚酸、黄酮和脂肪酰糖苷），对植物地上部分进行了分析。在来自不同栖息地的森林鼠尾草样品中，观察到植物化学成分和抗氧化活性存在显著差异。迷迭香酸、鼠尾草酸和咖啡酰奎宁酸（具有显著药理特性的重要多酚化合物，特别是在其抗氧化、抗炎和酶抑制活性方面）被确定为森林鼠尾草种群中的主要化合物。根据层次聚类分析（HCA），基于植物化学成分和抗氧化活性确定了三组物种。数据分析表明，化合物的质量和数量及其抗氧化功能因栖息地多样性而异。总体而言，结果表明每个种群都表现出独特的一类多酚，突出了其在各个行业的不同潜在应用。这些发现表明，这里研究的森林鼠尾草种群在开发创新营养保健品方面具有巨大潜力，可解决目前市场上森林鼠尾草衍生产品稀缺的问题。
