具有高药用价值的观赏植物(唇形科)毛萼香茶菜(D. Don)Rehd. 的完整叶绿体基因组。
The complete chloroplast genome of (D. Don) Rehd. (Labiatae), an ornamental plant with high medicinal value.
作者信息
Yu Xiao, Song Yu-Ru, Zhao Zhen-Ning
机构信息
School of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming, China.
School of Forestry, Southwest Forestry University, Kunming, China.
出版信息
Mitochondrial DNA B Resour. 2023 Feb 28;8(3):336-341. doi: 10.1080/23802359.2023.2183069. eCollection 2023.
is an ornamental plant with high medicinal value. In this study, we sequenced and analyzed the complete chloroplast (cp) genome of the species. The complete cp sequence is 151,550 bp, including the large single-copy (LSC) region of 82,778 bp, the small single-copy (SSC) region of 17,492 bp, and a pair of invert repeats (IRs) regions of 25,640 bp. It encodes 132 unique genes in total, including 87 protein-coding genes, 37 transfer RNA genes (tRNAs), and eight ribosomal RNA genes (rRNAs). The comparative analysis of complete cp genomes showed that the genomic structure and gene order of cps were conserved. The sequences of , , , , , , , , , , , , and serve as hotspots for developing the DNA barcoding of species. There are 49 SSR loci in the cp genome of , among which the repeat numbers of mononucleotide, dinucleotide, trinucleotide, tetranucleotide, and pentanucleotides SSR are 37, 9, 3, 0, and 0, respectively. A total of 50 repeats were detected, including 15 forward repeats, seven reverse repeats, 26 palindromic repeats, and two complementary repeats. Phylogenetic analysis based on the complete cp genome and protein-coding DNA sequences of 26 plants indicates that has a dose relationship with and .
是一种具有高药用价值的观赏植物。在本研究中,我们对该物种的完整叶绿体(cp)基因组进行了测序和分析。完整的cp序列为151,550 bp,包括82,778 bp的大单拷贝(LSC)区域、17,492 bp的小单拷贝(SSC)区域和一对25,640 bp的反向重复(IRs)区域。它总共编码132个独特基因,包括87个蛋白质编码基因、37个转运RNA基因(tRNAs)和8个核糖体RNA基因(rRNAs)。完整cp基因组的比较分析表明,cp的基因组结构和基因顺序是保守的。 、 、 、 、 、 、 、 、 、 、 、 和 的序列可作为开发该物种DNA条形码的热点区域。该物种的cp基因组中有49个简单序列重复(SSR)位点,其中单核苷酸、二核苷酸、三核苷酸、四核苷酸和五核苷酸SSR的重复次数分别为37、9、3、0和0。共检测到50个重复序列,包括15个正向重复序列、7个反向重复序列、26个回文重复序列和2个互补重复序列。基于26种植物的完整cp基因组和蛋白质编码DNA序列的系统发育分析表明, 与 和 存在剂量关系。