Department of Physics, Bio-Medical Physics Laboratory, Jordan University of Science and Technology, Jordan; School of Physics, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia; NanoBiotechnology Research and Innovation (NanoBRI), Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Medical Imaging and radiography Department, Aqaba University of Technology, Aqaba, Jordan.
Photodiagnosis Photodyn Ther. 2022 Jun;38:102801. doi: 10.1016/j.pdpdt.2022.102801. Epub 2022 Mar 11.
BACKGROUND/AIMS: Silica nanoparticles (SiNPs) have been promising vehicles for drug delivery. Cichorium Pumilum (CP), a natural photosensitizer (PS), has been reported to have many useful effects in cancer treatment. However, the poor water solubility and its low bioavailability have confined its use as a suitable photosensitizer for photodynamic therapy. Therefore, a subtle approach is required to overcome these drawbacks.
We have synthesized a silica nanoparticles loaded with Cichorium Pumilum. The nanoparticles structural morphologies have been charectrized by Transmission Electron Microscopy (TEM). The cytotoxicity for different concentrations of naked and encapsulated CP was evaluated. Moreover, the optimal concentration of naked and encapsulated CP with exposure time to a light (Maximum intensity at 350nm ∼0.27mW/cm) required to eliminate the used cells (Osteosarcoma cells) were also measured.
The results showed that encapsulated CP in SiNPs exhibited relatively higher efficacy than the naked CP by + 157.14 % of exposure time efficacy and + 49.45% of concentration efficacy, and encapsulated CP was also confirmed to be effective in eradicating osteosarcoma cells.
The engineered silica nanoparticles loaded with CP enhanced the photodynamic therapy by increasing the CP bioavailability.
背景/目的：二氧化硅纳米颗粒（SiNPs）已成为药物输送的有前途的载体。小蓟（CP），一种天然光敏剂（PS），已被报道在癌症治疗中有许多有用的作用。然而，其较差的水溶性和低生物利用度限制了其作为光动力疗法的合适光敏剂的应用。因此，需要采用巧妙的方法来克服这些缺点。
我们已经合成了负载小蓟的二氧化硅纳米颗粒。通过透射电子显微镜（TEM）对纳米颗粒的结构形态进行了表征。评估了不同浓度的裸 CP 和封装 CP 的细胞毒性。此外，还测量了消除使用的细胞（骨肉瘤细胞）所需的裸 CP 和封装 CP 的最佳浓度以及暴露于光（最大强度在 350nm 左右为 0.27mW/cm）的时间。
结果表明，封装在 SiNPs 中的 CP 比裸 CP 具有更高的疗效，暴露时间的疗效提高了 +157.14%，浓度的疗效提高了+49.45%，并且封装 CP 也被证实能够有效根除骨肉瘤细胞。
负载 CP 的工程化二氧化硅纳米颗粒通过提高 CP 的生物利用度增强了光动力疗法。
