鸟嘌呤和腺嘌呤类似物作为研究大肠杆菌错配修复机制的工具
Guanine and adenine analogues as tools in the investigation of the mechanisms of mismatch repair in E. coli.
作者信息
Wood S G, Ubasawa A, Martin D, Jiricny J
出版信息
Nucleic Acids Res. 1986 Aug 26;14(16):6591-602. doi: 10.1093/nar/14.16.6591.
Abstract
The efficiency of in vivo correction of five "mismatch analogues", incorporated into M13mp9 DNA, was studied in an attempt to elucidate the structural determinants required for mismatch recognition by the repair machinery of E. coli. Inosine was efficiently removed from an I/T mismatch, presumably by the action of hypoxanthine glycosylase. The mismatch analogues DI/T (DI = 7-deazainosine), Tu/C (Tu = tubercidin), N/C (N = nebularine) and DN/C (DN = 7-deazanebularine) were left largely unrepaired, giving rise to high yields of mutant phenotype. The efficiency of correction of these mismatch analogues could be correlated with their structure within the base-pair.
摘要
研究了掺入M13mp9 DNA中的五种“错配类似物”在体内的校正效率，以试图阐明大肠杆菌修复机制识别错配所需的结构决定因素。肌苷可能通过次黄嘌呤糖基化酶的作用从I/T错配中有效去除。错配类似物DI/T（DI = 7-脱氮肌苷）、Tu/C（Tu = 杀结核菌素）、N/C（N = 喷司他丁）和DN/C（DN = 7-脱氮喷司他丁）大部分未被修复，产生了高比例的突变表型。这些错配类似物的校正效率与其在碱基对中的结构相关。
相似文献
1
Guanine and adenine analogues as tools in the investigation of the mechanisms of mismatch repair in E. coli.鸟嘌呤和腺嘌呤类似物作为研究大肠杆菌错配修复机制的工具
Nucleic Acids Res. 1986 Aug 26;14(16):6591-602. doi: 10.1093/nar/14.16.6591.
2
Adenine excisional repair function of MYH protein on the adenine:8-hydroxyguanine base pair in double-stranded DNA.MYH蛋白对双链DNA中腺嘌呤：8-羟基鸟嘌呤碱基对的腺嘌呤切除修复功能。
Nucleic Acids Res. 2000 Dec 15;28(24):4912-8. doi: 10.1093/nar/28.24.4912.
3
Influence of GATC sequences on Escherichia coli DNA mismatch repair in vitro.GATC序列对体外大肠杆菌DNA错配修复的影响。
J Bacteriol. 1987 Mar;169(3):1254-9. doi: 10.1128/jb.169.3.1254-1259.1987.
4
Some mismatch repair activities in Escherichia coli.大肠杆菌中的一些错配修复活性。
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9674-8. doi: 10.1073/pnas.85.24.9674.
5
Escherichia coli MutY protein has a guanine-DNA glycosylase that acts on 7,8-dihydro-8-oxoguanine:guanine mispair to prevent spontaneous G:C-->C:G transversions.大肠杆菌MutY蛋白具有一种鸟嘌呤-DNA糖基化酶，该酶作用于7,8-二氢-8-氧代鸟嘌呤与鸟嘌呤的错配，以防止自发的G:C→C:G颠换。
Nucleic Acids Res. 1998 Oct 15;26(20):4669-75. doi: 10.1093/nar/26.20.4669.
6
Structure-Activity Relationships Reveal Key Features of 8-Oxoguanine: A Mismatch Detection by the MutY Glycosylase.构效关系揭示8-氧代鸟嘌呤的关键特征：MutY糖基化酶的错配检测
ACS Chem Biol. 2017 Sep 15;12(9):2335-2344. doi: 10.1021/acschembio.7b00389. Epub 2017 Aug 8.
7
Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.大肠杆菌的甲基化导向DNA错配修复系统对不同的碱基/碱基错配进行校正的效率不同。
Cell. 1984 Oct;38(3):879-87. doi: 10.1016/0092-8674(84)90283-6.
8
Repair of A/G and A/8-oxoG mismatches by MutY adenine DNA glycosylase.MutY腺嘌呤DNA糖基化酶对A/G和A/8-氧代鸟嘌呤错配的修复
Methods Mol Biol. 2000;152:3-16. doi: 10.1385/1-59259-068-3:3.
9
Cellular defense mechanisms against alkylation of DNA.细胞针对DNA烷基化的防御机制。
Basic Life Sci. 1982;20:89-102. doi: 10.1007/978-1-4613-3476-7_4.
10
Identification of high excision capacity for 5-hydroxymethyluracil mispaired with guanine in DNA of Escherichia coli MutM, Nei and Nth DNA glycosylases.鉴定大肠杆菌MutM、Nei和Nth DNA糖基化酶对与鸟嘌呤错配的5-羟甲基尿嘧啶具有高切除能力。
Nucleic Acids Res. 2003 Feb 15;31(4):1191-6. doi: 10.1093/nar/gkg223.
引用本文的文献
1
Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches.错配结合蛋白MutS对含有不同错配的异源双链体的亲和力。
Biochem J. 2001 Mar 15;354(Pt 3):627-33. doi: 10.1042/0264-6021:3540627.
2
Structural features and hydration of a dodecamer duplex containing two C.A mispairs.包含两个C.A错配的十二聚体双链体的结构特征与水合作用
Nucleic Acids Res. 1987 Aug 25;15(16):6589-606. doi: 10.1093/nar/15.16.6589.
3
d(GATC) sequences influence Escherichia coli mismatch repair in a distance-dependent manner from positions both upstream and downstream of the mismatch.d(GATC)序列以与错配上下游位置距离相关的方式影响大肠杆菌错配修复。
Nucleic Acids Res. 1988 Jun 10;16(11):4875-90. doi: 10.1093/nar/16.11.4875.
4
Mismatch-containing oligonucleotide duplexes bound by the E. coli mutS-encoded protein.由大肠杆菌mutS编码蛋白结合的含错配寡核苷酸双链体。
Nucleic Acids Res. 1988 Aug 25;16(16):7843-53. doi: 10.1093/nar/16.16.7843.
5
Nature of the SOS mutator activity: genetic characterization of untargeted mutagenesis in Escherichia coli.SOS诱变活性的本质：大肠杆菌中非靶向诱变的遗传特征分析
Mol Gen Genet. 1988 Aug;213(2-3):491-8. doi: 10.1007/BF00339621.
6
Effect of uracil situated in the vicinity of a mispair on the directionality of mismatch correction in Escherichia coli.位于错配附近的尿嘧啶对大肠杆菌错配校正方向性的影响。
Nucleic Acids Res. 1991 Apr 11;19(7):1443-7. doi: 10.1093/nar/19.7.1443.
本文引用的文献
1
Repair of defined single base-pair mismatches in Escherichia coli.大肠杆菌中定义的单个碱基对错配的修复。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):503-5. doi: 10.1073/pnas.82.2.503.
2
Structure, dynamics, and energetics of deoxyguanosine . thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution.溶液中自互补双链体d(CGTGAATTCGCG) 中脱氧鸟苷-胸苷摆动碱基对形成的结构、动力学和能量学
Biochemistry. 1982 Feb 2;21(3):437-44. doi: 10.1021/bi00532a003.
3
Repair of DNA base-pair mismatches in extracts of Escherichia coli.大肠杆菌提取物中DNA碱基对错配的修复
Cold Spring Harb Symp Quant Biol. 1984;49:589-96. doi: 10.1101/sqb.1984.049.01.066.
4
Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.大肠杆菌的甲基化导向DNA错配修复系统对不同的碱基/碱基错配进行校正的效率不同。
Cell. 1984 Oct;38(3):879-87. doi: 10.1016/0092-8674(84)90283-6.
5
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.克隆至M13载体的DNA片段的寡核苷酸定向诱变
Methods Enzymol. 1983;100:468-500. doi: 10.1016/0076-6879(83)00074-9.
6
Fidelity of DNA synthesis.DNA合成的保真度。
Annu Rev Biochem. 1982;51:429-57. doi: 10.1146/annurev.bi.51.070182.002241.
7
Kinetic basis of spontaneous mutation. Misinsertion frequencies, proofreading specificities and cost of proofreading by DNA polymerases of Escherichia coli.自发突变的动力学基础。大肠杆菌DNA聚合酶的错配插入频率、校对特异性及校对成本。
J Mol Biol. 1982 Mar 25;156(1):37-51. doi: 10.1016/0022-2836(82)90457-0.
8
The gapped duplex DNA approach to oligonucleotide-directed mutation construction.用于寡核苷酸定向突变构建的缺口双链DNA方法。
Nucleic Acids Res. 1984 Dec 21;12(24):9441-56. doi: 10.1093/nar/12.24.9441.
9
High-resolution structure of a DNA helix containing mismatched base pairs.包含错配碱基对的DNA螺旋的高分辨率结构。
Nature. 1985;315(6020):604-6. doi: 10.1038/315604a0.
10
Structure of an adenine-cytosine base pair in DNA and its implications for mismatch repair.DNA中腺嘌呤-胞嘧啶碱基对的结构及其对错配修复的影响。
Nature. 1986;320(6062):552-5. doi: 10.1038/320552a0.
Zotero 插件