Wang Lihua, Tu Wenmiao, Jin Peng, Liu Yanlong, Du Junli, Zheng Jiacheng, Wang Yi-Hong, Li Jieqin
College of Agriculture, Anhui Science and Technology University, Fengyang, Anhui, China.
Anhui Province International Joint Research Center of Forage Bio-breeding, Chuzhou, China.
Front Plant Sci. 2024 Apr 10;15:1320844. doi: 10.3389/fpls.2024.1320844. eCollection 2024.
Sorghum plant color is the leaf sheath/leaf color and is associated with seed color, tannin and phenol content, head blight disease incidence, and phytoalexin production.
In this study, we evaluated plant color of the sorghum mini core collection by scoring leaf sheath/leaf color at maturity as tan, red, or purple across three testing environments and performed genome-wide association mapping (GWAS) with 6,094,317 SNPs markers.
Eight loci, one each on chromosomes 1, 2, 4, and 6 and two on chromosomes 5 and 9, were mapped. All loci contained one to three candidate genes. In , Sobic.005G165632 and Sobic.005G165700 were located in the same linkage disequilibrium (LD) block. In , Sobic.006G149650 and Sobic.006G149700 were located in the different LD block. The single peak in covered one gene, Sobic.006G149700, which was a senescence regulator. We found a loose correlation between the degree of linkage and tissue/organ expression of the underlying genes possibly related to the plant color phenotype. Allele analysis indicated that none of the linked SNPs can differentiate between red and purple accessions whereas all linked SNPs can differentiate tan from red/purple accessions. The candidate genes and SNP markers may facilitate the elucidation of plant color development as well as molecular plant breeding.
高粱植株颜色即叶鞘/叶片颜色，与种子颜色、单宁和酚类含量、穗腐病发病率以及植保素产生相关。
在本研究中，我们通过在三个测试环境中对成熟时叶鞘/叶片颜色评分为棕褐色、红色或紫色，评估了高粱微型核心种质的植株颜色，并使用6,094,317个单核苷酸多态性（SNP）标记进行全基因组关联图谱分析（GWAS）。
共定位到8个位点，分别位于第1、2、4和6号染色体上各1个，第5和9号染色体上各2个。所有位点均包含1至3个候选基因。在[具体情况未明确处1]中，Sobic.005G165632和Sobic.005G165700位于同一连锁不平衡（LD）块中。在[具体情况未明确处2]中，Sobic.006G149650和Sobic.006G149700位于不同的LD块中。[具体情况未明确处3]中的单峰覆盖了一个基因Sobic.006G149700，它是一个衰老调节因子。我们发现潜在基因的连锁程度与可能与植株颜色表型相关的组织/器官表达之间存在松散的相关性。等位基因分析表明，没有一个连锁SNP能够区分红色和紫色种质，而所有连锁SNP都能区分棕褐色与红色/紫色种质。这些候选基因和SNP标记可能有助于阐明植株颜色发育以及分子植物育种。
