Wang Ying, Wang Geng, Zhang Lulu, Ren Lili, Wang Jianwei
NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing 100730, China.
Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Biosaf Health. 2022 Apr;4(2):66-69. doi: 10.1016/j.bsheal.2022.03.007. Epub 2022 Mar 11.
Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling quality. We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) retrieved from the swabs using the RT-PCR method. Significant different concentrations of GAPDH were detected, ranged from 4.36 × 10 copies/mL to 6.98 × 10 copies/mL among the five swabs ( < 0.05). The designation of the swab head, with or without tip expansion, had limited influence on the collection efficiency. The discrepancy among the NPSs emphasized the improvement of the swab head material.
上呼吸道样本是2019冠状病毒病（COVID-19）诊断中最常用的样本。从鼻咽部采集的样本更适合用于病毒核酸检测。商用鼻咽拭子（NPS）是影响采样质量的主要因素。我们通过使用逆转录聚合酶链反应（RT-PCR）方法检测从拭子中获取的甘油醛-3-磷酸脱氢酶基因（GAPDH）的浓度，评估了来自五个制造商的NPS的可接受性和效率。在五种拭子中检测到GAPDH浓度存在显著差异，范围为4.36×10拷贝/毫升至6.98×10拷贝/毫升（P<0.05）。拭子头部是否有尖端扩张对采集效率的影响有限。NPS之间的差异强调了拭子头部材料的改进。
