长链非编码 RNA PTCSC3 在骨质疏松症中上调，并负调控成骨细胞凋亡。
LncRNA PTCSC3 is upregulated in osteoporosis and negatively regulates osteoblast apoptosis.
机构信息
Orthopedics Department, Hebei Yanda Hospital, Yanjiao Development Zone, Sanhe City, Langfang City, 065201, Hebei Province, People's Republic of China.
Orthopedics Department, Beijing Chao-Yang Hospital, Capital Medical University, No 8 Gongtinan Road, Beijing, 100020, People's Republic of China.
出版信息
BMC Med Genomics. 2022 Mar 16;15(1):57. doi: 10.1186/s12920-022-01182-3.
BACKGROUND
It is known that long non-coding RNA (lncRNA) PTCSC3 is involved in thyroid cancer and glioma, but its function in osteoporosis is unknown. The aim of our study was to investigate the role of lncRNA PTCSC3 in osteoporosis.
METHODS
A total of 80 patients with osteoporosis (4 clinical stages) and four corresponding groups of healthy controls were enrolled. Plasma PTCSC3 levels in the 80 osteoporosis patients and 80 healthy volunteers were measured using RT-qPCR. The diagnostic potential of plasma PTCSC3 for osteoporosis was evaluated by ROC curve analysis with healthy volunteers as the true negative cases and corresponding osteoporosis patients as the true positive cases.
RESULTS
PTCSC3 was upregulated in osteoporosis patients compared with healthy controls. PTCSC3 levels increased with osteoporosis stages increasing, but not with healthy controls aging. PTCSC3 overexpression separated each stage of osteoporosis from corresponding controls. PTCSC3 overexpression promoted while PTCSC3 silencing inhibited osteoblast apoptosis. However, PTCSC3 overexpression and silencing showed no significant effect on osteoclast apoptosis. LncRNA PTCSC3 was upregulated in osteoporosis and negatively regulated osteoblast apoptosis.
CONCLUSION
LncRNA PTCSC3 may serve as a potential therapeutic target for osteoporosis.
背景
长链非编码 RNA（lncRNA）PTCSC3 参与甲状腺癌和神经胶质瘤，但它在骨质疏松症中的作用尚不清楚。本研究旨在探讨 lncRNA PTCSC3 在骨质疏松症中的作用。
方法
共纳入 80 例骨质疏松症（4 个临床阶段）患者和 4 组相应的健康对照者。采用 RT-qPCR 检测 80 例骨质疏松症患者和 80 例健康志愿者的血浆 PTCSC3 水平。以健康志愿者为真阴性病例，相应的骨质疏松症患者为真阳性病例，通过 ROC 曲线分析评估血浆 PTCSC3 对骨质疏松症的诊断潜力。
结果
与健康对照组相比，骨质疏松症患者中 PTCSC3 上调。PTCSC3 水平随骨质疏松症阶段的增加而增加，但与健康对照组的年龄无关。PTCSC3 过表达将每个骨质疏松症阶段与相应的对照组分开。PTCSC3 过表达促进而 PTCSC3 沉默抑制成骨细胞凋亡。然而，PTCSC3 过表达和沉默对破骨细胞凋亡没有明显影响。lncRNA PTCSC3 在骨质疏松症中上调并负调控成骨细胞凋亡。
结论
lncRNA PTCSC3 可能是骨质疏松症的潜在治疗靶点。
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