Department of Pathology, Shaoxing Hospital of Traditional Chinese Medicine, Shaoxing, Zhejiang, P.R. China.
J Cell Biochem. 2019 Sep;120(9):15083-15088. doi: 10.1002/jcb.28769. Epub 2019 Apr 26.
Long noncoding RNA (lncRNA) PTCSC3 (hereafter PTCSC3 is used to represent lncRNA PTCSC3) inhibits glioma and thyroid cancer, indicating its potential tumor suppression function in other types of cancers. We explored the potential involvement of PTCSC3 in triple-negative breast cancer (TNBC). In the current study, we found that PTCSC3 was downregulated in tumor tissues of patients with TNBC. PTCSC3 expression was positively correlated with plasma levels of PTCSC3. LncRNA H19 was upregulated and was inversely correlated with PTCSC3 in tumor tissues. PTCSC3 overexpression led to downregulated H19 in TNBC cells, while H19 overexpression did not affect PTCSC3 expression. PTCSC3 inhibited and H19 promoted proliferation of TNBC cells. H19 overexpression attenuated the effects of PTCSC3 overexpression. Cancer cell migration and invasion were not significantly affected by PTCSC3 overexpression. Therefore, lncRNA PTCSC3 inhibits TNBC cell proliferation by downregulating lncRNA H19.
长链非编码 RNA (lncRNA) PTCSC3（以下简称 PTCSC3 用于代表 lncRNA PTCSC3）抑制神经胶质瘤和甲状腺癌，表明其在其他类型癌症中具有潜在的肿瘤抑制功能。我们探讨了 PTCSC3 参与三阴性乳腺癌 (TNBC) 的潜在作用。在本研究中，我们发现 PTCSC3 在 TNBC 患者的肿瘤组织中表达下调。PTCSC3 的表达与 PTCSC3 的血浆水平呈正相关。在肿瘤组织中，lncRNA H19 上调且与 PTCSC3 呈负相关。PTCSC3 过表达导致 TNBC 细胞中 H19 下调，而 H19 过表达不影响 PTCSC3 的表达。PTCSC3 抑制 TNBC 细胞的增殖，而 H19 促进 TNBC 细胞的增殖。H19 过表达减弱了 PTCSC3 过表达的作用。PTCSC3 过表达对癌细胞的迁移和侵袭没有显著影响。因此，lncRNA PTCSC3 通过下调 lncRNA H19 抑制 TNBC 细胞的增殖。
