The Key Laboratory of Animal Nutrition and Feed Science in East China of Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Hainan Institute of Zhejiang University, Yazhou Bay Science and Technology City, Zhejiang University, Sanya, Hainan 572025, China.
J Anim Sci. 2022 May 1;100(5). doi: 10.1093/jas/skac088.
To explore the effect of coated tannin (CT) on the growth performance, nutrients digestibility, and intestinal function in weaned piglets, a total of 180 piglets Duroc × Landrace × Yorkshire (28 d old) weighing about 8.6 kg were randomly allotted to three treatments: 1) Con: basal diet (contains ZnSO4); 2) Tan: basal diet + 0.15% CT; and 3) ZnO: basal diet + ZnO (Zn content is 1,600 mg/kg). The results showed that 0.15% CT could highly increase the average daily gain and average daily feed intake of weaned piglets compared with the control group, especially decreasing diarrhea incidence significantly (P < 0.05). Compared with the control group, crude protein apparent digestibility and digestive enzyme activity of the piglets fed with 0.15% CT were enhanced obviously (P < 0.05). Meanwhile, the intestinal villi and microvilli arranged more densely, while the content of serum diamine oxidase was decreased, and the protein expressions of zonula occludens-1 (ZO-1) and claudin-1 were significantly upregulated (P < 0.05). In addition, CT altered the structure of intestinal microbiota and augmented some butyrate-producing bacteria such as Ruminococcaceae and Megasphaera. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis also showed that the abundances of pathways related to butyrate metabolism and tryptophan metabolism were increased; however, the function of lipopolysaccharide biosynthesis proteins was significantly decreased. The results demonstrated that 0.15% CT could improve growth performance, digestibility, and intestinal function of weaned piglets, and it had the potential to replace ZnO applied to farming.
为了探究包被单宁（CT）对断奶仔猪生长性能、养分消化率和肠道功能的影响，本试验选用大约 8.6kg 的杜洛克×长白×约克夏三元杂交断奶仔猪 180 头，随机分为 3 个处理组：1）对照组：基础饲粮（含 ZnSO4）；2）单宁组：基础饲粮+0.15%CT；3）氧化锌组：基础饲粮+ZnO（Zn 含量为 1600mg/kg）。结果表明：与对照组相比，饲粮添加 0.15%CT 可显著提高断奶仔猪的平均日增重和平均日采食量，且仔猪腹泻率显著降低（P<0.05）。与对照组相比，饲粮添加 0.15%CT 可显著提高仔猪粗蛋白表观消化率和消化酶活性（P<0.05）。同时，仔猪的肠绒毛和微绒毛排列更加紧密，血清二胺氧化酶含量降低，紧密连接蛋白-1（ZO-1）和 Claudin-1 的蛋白表达显著上调（P<0.05）。此外，CT 改变了肠道微生物群落的结构，增加了一些丁酸产生菌，如瘤胃球菌科和巨球形菌。PICRUSt（通过重建未观察状态来探究群落的系统发育）分析也表明，与丁酸代谢和色氨酸代谢相关的途径丰度增加，而脂多糖生物合成蛋白的功能显著降低。结果表明，饲粮添加 0.15%CT 可提高断奶仔猪的生长性能、消化率和肠道功能，具有替代饲粮中 ZnO 的潜力。
