Sajadi Seyede Sogand, Haniloo Ali, Nadri Samad, Torabi Negin
Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Mahdavi Blvd., Shahrak Karmandan, P.O. Box 45139-56111, Zanjan, Iran.
Department of Medical Nanotechnology, School of Medicine, ZUMS, Zanjan, Iran.
J Parasit Dis. 2022 Mar;46(1):133-138. doi: 10.1007/s12639-021-01427-5. Epub 2021 Aug 2.
-developed metacestodes in the cultured medium are used for the assessment of its susceptibility to different compounds; however, this procedure is time-consuming and risky. In the present study, aspirated protoscoleces from the infected sheep were used to evaluate the effects of glucose, as an energy source, as well as ascorbic acid, as an antioxidant vitamin, on larval development. Protoscoleces were maintained in RPMI culture media containing 10% fetal calf serum, as well as different concentrations of glucose (4, 6, and 8 mg/ml) and ascorbic acid (25, 50, and 100 µg/ml). A culture medium containing 4 mg/ml of glucose was served as the control. Larger cysts were achieved in a shorter time from the medium enriched with 6 mg/ml of glucose (740 ± 20 µm) compared to the control group (420 ± 40 µm). However, in the groups treated with ascorbic acid, the number of cysts was higher in 100 µg/ml (32.5 ± 0.7) compared to the control group (12.5 ± 0.7). Additionally, the mature cysts were achieved on the 7th day of cultivation with 100 µg/ml of ascorbic acid compared to 18 days in the control group.
