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单细胞转录组学揭示了小鼠乳腺成纤维细胞的异质性，这些细胞具有不同的功能、雌激素反应、分化过程以及与上皮细胞的相互作用。

Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium.

作者信息

Yoshitake Ryohei, Chang Gregory, Saeki Kohei, Ha Desiree, Wu Xiwei, Wang Jinhui, Chen Shiuan

机构信息

Department of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United States.

Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Japan.

出版信息

Front Cell Dev Biol. 2022 Mar 1;10:850568. doi: 10.3389/fcell.2022.850568. eCollection 2022.

DOI:10.3389/fcell.2022.850568
PMID:35300413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8923650/
Abstract

Fibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types of mouse mammary gland fibroblasts through single-cell RNA sequencing analysis: fibroblasts and fibroblasts. Each population exhibits unique functional characteristics as well as discrete localization in normal mouse mammary glands. Remarkably, estrogen, a crucial mediator of mammary gland organization, alters the gene expression profiles of fibroblasts in a population-specific manner, without distinct activation of estrogen receptor signaling. Further integrative analysis with the inclusion of five other publicly available datasets reveals a directional differentiation among the mammary gland fibroblast populations. Moreover, the combination with the mouse mammary epithelium atlas allows us to infer multiple potential interactions between epithelial cells and fibroblasts in mammary glands. This study provides a comprehensive view of mouse mammary gland fibroblasts at the single-cell level.

摘要

成纤维细胞已被证明是乳腺组织形成的关键参与者之一。在此，我们通过单细胞RNA测序分析确定了小鼠乳腺成纤维细胞的两种主要类型： 成纤维细胞和 成纤维细胞。每个群体在正常小鼠乳腺中都表现出独特的功能特征以及离散的定位。值得注意的是，雌激素是乳腺组织形成的关键介质，它以群体特异性方式改变成纤维细胞的基因表达谱，而不会明显激活雌激素受体信号。进一步纳入其他五个公开可用数据集的综合分析揭示了乳腺成纤维细胞群体之间的定向分化。此外，与小鼠乳腺上皮图谱相结合使我们能够推断乳腺上皮细胞和成纤维细胞之间的多种潜在相互作用。这项研究在单细胞水平上提供了小鼠乳腺成纤维细胞的全面视图。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/d867621ebe23/fcell-10-850568-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/d4c172dd5c72/fcell-10-850568-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/6bc80bd5a4d0/fcell-10-850568-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cb0ebb5ba3eb/fcell-10-850568-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/00776d091ba2/fcell-10-850568-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cb3e6b8045da/fcell-10-850568-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cd2bc13ba011/fcell-10-850568-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/d867621ebe23/fcell-10-850568-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/d4c172dd5c72/fcell-10-850568-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/6bc80bd5a4d0/fcell-10-850568-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cb0ebb5ba3eb/fcell-10-850568-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/00776d091ba2/fcell-10-850568-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cb3e6b8045da/fcell-10-850568-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/cd2bc13ba011/fcell-10-850568-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e77/8923650/d867621ebe23/fcell-10-850568-g007.jpg

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