Arstila P, Vuorimaa T, Kalimo K, Halonen P, Viljanen M, Granfors K, Toivanen P
J Gen Virol. 1977 Jan;34(1):167-76. doi: 10.1099/0022-1317-34-1-167.
A solid-phase radioimmunoassay (RIA) was used to determine the presence of IgG and IgM antibodies to measles virus in human serum and cerebrospinal fluid (CSF). Purified measles virus was adsorbed on to polystyrene balls, which were then exposed to serial dilutions of test serum or CSF. The presence of antibody was measured by its capacity to bind 125I-labelled specific anti-human IgG or IgM. Serum from a variety of patients as well as measles-immune clinically healthy persons were tested; binding ratios (using negative human serum controls) were usually between 10 and 30, but with subacute sclerosing panencephalitis (SSPE) ratios were as high as 50. Of ten CSF specimens tested, all but one, which was taken early in the convalescent phase of measles infection, had detectable IgG antibody. In six patients with acute measles, IgM antibodies were found in all serum specimens taken one or more days after the onset of rash. Maximal titers of 1:10000 to 1:40000 were found about 7 days later. Thereafter, IgM titres decreased rapidly but were still detectable at 40 days. A purified ribonucleoprotein of measles virus was also used successfully as an antigen in this RIA method.
采用固相放射免疫测定法（RIA）检测人血清和脑脊液（CSF）中针对麻疹病毒的IgG和IgM抗体。将纯化的麻疹病毒吸附到聚苯乙烯球上，然后将其暴露于系列稀释的待测血清或脑脊液中。通过其结合125I标记的特异性抗人IgG或IgM的能力来检测抗体的存在。对各类患者以及临床上健康的麻疹免疫者的血清进行了检测；结合率（使用人阴性血清对照）通常在10至30之间，但亚急性硬化性全脑炎（SSPE）患者的结合率高达50。在所检测的10份脑脊液标本中，除1份在麻疹感染恢复期早期采集的标本外，其余均检测到可检测到的IgG抗体。在6例急性麻疹患者中，所有出疹后1天或更长时间采集的血清标本中均发现了IgM抗体。约7天后发现最大滴度为1:10000至1:40000。此后，IgM滴度迅速下降，但在40天时仍可检测到。麻疹病毒的纯化核糖核蛋白也成功用作该RIA方法中的抗原。
