一种基于引物交换反应的 SARS-CoV-2 RNA 快速检测方法。
A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction.
机构信息
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China.
Department of Neurology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, P. R. China.
出版信息
Chem Commun (Camb). 2022 Apr 5;58(28):4484-4487. doi: 10.1039/d2cc00488g.
A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.
本工作提出了一种基于引物交换反应（PER）的 SARS-CoV-2 RNA 检测的简单方法。通过巧妙地整合 PER 级联和 CRISPR/cas12a 系统，该方法可以在 40 分钟内方便地检测目标 RNA，并能区分目标序列中的单碱基突变，表现出优异的分析性能。
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