控制对烷化剂敏感性的大肠杆菌基因。
Escherichia coli gene that controls sensitivity to alkylating agents.
作者信息
Yamamoto Y, Katsuki M, Sekiguchi M, Otsuji N
出版信息
J Bacteriol. 1978 Jul;135(1):144-52. doi: 10.1128/jb.135.1.144-152.1978.
Abstract
A new type of Escherichia coli mutant which shows increased sensitivity to methyl methane sulfonate but not to UV light or to gamma rays was isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The mutant is unable to reactivate phage lambdavir or double-stranded phiX174 DNA (replicative form) that had been treated with methyl methane sulfonate. The mutant is sensitive to other alkylating agents, such as ethyl methane sulfonate, mitomycin C, and N-methyl-N'-nitro-N-nitrosoguanidine, as well. It grows normally and exhibits almost normal recombination proficiency. The mutant possesses normal levels of DNA polymerase I, exonuclease I, exonuclease V, endonuclease specific for methyl methane sulfonate-treated DNA, and 3-methyladenine-DNA glycosidase activities. The genetic locus responsible has been named alk and is located near his on the chromosome.
摘要
用N-甲基-N'-硝基-N-亚硝基胍诱变后，分离出一种新型大肠杆菌突变体，该突变体对甲磺酸甲酯敏感性增加，但对紫外线或γ射线不敏感。该突变体无法重新激活经甲磺酸甲酯处理过的λ噬菌体或双链φX174 DNA（复制型）。该突变体对其他烷化剂也敏感，如甲磺酸乙酯、丝裂霉素C和N-甲基-N'-硝基-N-亚硝基胍。它生长正常，重组能力几乎正常。该突变体的DNA聚合酶I、核酸外切酶I、核酸外切酶V、对甲磺酸甲酯处理的DNA具有特异性的核酸内切酶以及3-甲基腺嘌呤-DNA糖苷酶活性水平正常。负责该性状的基因座已被命名为alk，位于染色体上his附近。
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