Institute Technology of Sepuluh Nopember, Chemical Engineering Department, Surabaya, Indonesia.
Natural Products Laboratory, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Planta Med. 2022 Aug;88(9-10):814-825. doi: 10.1055/a-1803-3259. Epub 2022 Mar 18.
The isolation of a compound from a natural source involves many organic and mostly toxic solvents for extraction and purification. Natural deep eutectic solvents have been shown to be efficient options for the extraction of natural products. They have the advantage of being composed of abundantly available common primary metabolites, being nontoxic and environmentally safe solvents. The aim of this study was to develop a natural deep eutectic solvent-based extraction method for galanthamine, an important therapeutic agent for the treatment of Alzheimer's disease. This alkaloid can be produced by synthesis or by extraction from bulbs. To develop an efficient extraction method, a number of different natural deep eutectic solvents was first tested for their solubilization capacity of galanthamine bromide salt. Promising results were obtained for ionic liquids, as well as some amphoteric and acidic natural deep eutectic solvents. In a two-cycle extraction process, the best solvents were tested for the extraction of galanthamine from bulbs. The ionic liquids produced poor yields, and the best results were obtained with some acid and sugar mixtures, among which malic acid-sucrose-water (1 : 1 : 5) proved to be the best, showing similar yields to that of the exhaustive Soxhlet extraction with methanol. Furthermore, the natural deep eutectic solvent was more selective for galanthamine.
从天然来源中分离化合物涉及许多用于提取和纯化的有机且大多有毒的溶剂。天然深共晶溶剂已被证明是提取天然产物的有效选择。它们具有由丰富的常见初级代谢物组成、无毒和环境安全溶剂的优点。本研究旨在开发一种基于天然深共晶溶剂的提取方法，用于提取石蒜碱，这是治疗阿尔茨海默病的重要治疗剂。这种生物碱可以通过合成或从鳞茎中提取来生产。为了开发一种有效的提取方法，首先测试了许多不同的天然深共晶溶剂对石蒜碱溴化物盐的溶解能力。离子液体以及一些两性和酸性天然深共晶溶剂的结果很有前景。在两轮提取过程中，测试了最佳溶剂从鳞茎中提取石蒜碱。离子液体产率较低，而一些酸和糖混合物的产率最高，其中苹果酸-蔗糖-水（1：1：5）效果最好，与甲醇索氏提取的产率相似。此外，天然深共晶溶剂对石蒜碱的选择性更高。
