Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Asian Pac J Cancer Prev. 2020 May 1;21(5):1311-1315. doi: 10.31557/APJCP.2020.21.5.1311.
This study aimed to evaluate the expression patterns of miR-103, miR-184, miR-378, miR497 and in squamous cell carcinoma (SCC) of the tongue and to be compared with normal peripheral tissues.
Tumor and marginal tissues were obtained from 50 patients with OSCC. After RNA extraction, expression level of miR-103, miR-184, miR-378, miR497, and miR506 was estimated using SYBR green master mix and real-time quantitative PCR.
It was observed that, there was no detectable difference in expression level of miR-103 between tumoral and marginal tissues. However, expression level of miR-184, and miR-378 showed significant increase in tumor tissue samples compared to marginal tissue samples. MiR-497 and miR-506 demonstrated considerable decrease in tumoral cells in comparison with peripheral tissues. Moreover, the expression level of miRNAs was associated with clinicopathological features of the patients.
Our data indicated that miR-184, miR-378, miR-497, and miR-506 can be used as a potential diagnostic and prognostic biomarker in OSCC. Nevertheless, more studies are needed to confirm this claim.
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本研究旨在评估 miR-103、miR-184、miR-378、miR497 和 miR506 在舌鳞状细胞癌（SCC）中的表达模式，并与正常外周组织进行比较。
方法：从 50 例 OSCC 患者中获取肿瘤和边缘组织。提取 RNA 后，使用 SYBR 绿色主混合物和实时定量 PCR 估计 miR-103、miR-184、miR-378、miR497 和 miR506 的表达水平。
结果：观察到，miR-103 在肿瘤组织和边缘组织之间的表达水平没有差异。然而，与边缘组织样本相比，miR-184 和 miR-378 在肿瘤组织样本中的表达水平显著增加。与外周组织相比，miR-497 和 miR-506 在肿瘤细胞中的表达水平明显降低。此外，miRNAs 的表达水平与患者的临床病理特征相关。
结论：我们的数据表明，miR-184、miR-378、miR-497 和 miR-506 可以作为 OSCC 的潜在诊断和预后生物标志物。然而，需要更多的研究来证实这一说法。
