Seoul Metropolitan Government Research Institute of Public Health and Environment, Gyeonggi-do, Republic of Korea.
Lett Appl Microbiol. 2022 Jul;75(1):29-35. doi: 10.1111/lam.13702. Epub 2022 Mar 29.
Noroviruses cause acute gastroenteritis with symptoms of diarrhoea and vomiting, and their high infectivity allows outbreaks to readily occur. Quickly identifying and isolating potential contaminants is an effective method to prevent the spread of outbreaks. A total of 376 samples collected from nine outbreaks were categorized as either patient, asymptomatic individual, cook or environmental samples, according to the source of contamination. Using real-time PCR and sequencing analysis, norovirus GII genotypes were detected in 34·9% of samples from patients, 19·2% from asymptomatic individuals, 2·4% from the environment and 1·4% from cooks. Our findings showed contrasting results in samples categories quantified based on the limit of blank and detection limit by reverse transcription droplet digital PCR, which is a more sensitive testing method than real-time-PCR.
诺如病毒可引起腹泻和呕吐为特征的急性肠胃炎，其高传染性使得疫情很容易发生。快速识别和隔离潜在的污染物是预防疫情传播的有效方法。根据污染源的不同，将从 9 起暴发中采集的 376 份样本分为患者、无症状个体、厨师或环境样本。通过实时 PCR 和测序分析，在患者样本中检测到诺如病毒 GII 基因型占 34.9%，在无症状个体样本中检测到 19.2%，在环境样本中检测到 2.4%，在厨师样本中检测到 1.4%。我们的发现显示，基于空白限制和逆转录液滴数字 PCR 检测限的样本分类定量结果存在差异，逆转录液滴数字 PCR 是比实时 PCR 更敏感的检测方法。
