School of Medicine, Tongji University, Shanghai 200092, China; Department of Medical Oncology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China.
SJTU-Yale Joint Center for Biostatistics and Data Science, Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Sci Total Environ. 2022 Jul 10;829:154652. doi: 10.1016/j.scitotenv.2022.154652. Epub 2022 Mar 18.
Smoking is a serious public health problem that affects human health conditions. Although there is evidence that microorganisms are associated with smoking-related lung diseases, the relationship between the rich lung microbiome of upper respiratory tract groups and smoking has not been studied.
In this study, we investigated the effects of smoking on environmental microbes and lung microbiome in the Chinese population and provided clues for the role of smoking in the development of respiratory disease.
Bronchoalveolar lavage fluid samples were collected from 55 individuals with a history of smoking. Microbial gene sequencing was carried out through NGS technology. We analyzed and compared the diversity, community structure, and species abundance of bronchoalveolar lavage microbiome between smokers and nonsmokers, to speculate the effects of smoking on the lung microbiome.
Smoking hardly affected the α diversity of microbial groups of bronchoalveolar lavage, but it had a huge influence on the microbiome composition. The relative abundance of Rothia, Actinomycetes, Haemophilus, Porphyrins, Neisseria, Acinetobacter, and Streptococcus genera had a remarkable increase in the smoking group. On the other hand, the relative abundance of Plusella and Veronella decreased significantly.
Smoking may change the environmental microbes and then alter the structure of the lung microbiome, which may lead to smoking-related diseases.
吸烟是一个严重的公共卫生问题，影响人类健康状况。虽然有证据表明微生物与吸烟相关的肺部疾病有关，但上呼吸道群体丰富的肺部微生物群与吸烟之间的关系尚未得到研究。
本研究调查了吸烟对中国人群环境微生物和肺部微生物组的影响，为吸烟在呼吸道疾病发展中的作用提供了线索。
采集 55 名有吸烟史个体的支气管肺泡灌洗液样本。通过 NGS 技术进行微生物基因测序。我们分析和比较了吸烟者和非吸烟者支气管肺泡灌洗液微生物组的多样性、群落结构和物种丰度，以推测吸烟对肺部微生物组的影响。
吸烟几乎不会影响支气管肺泡灌洗微生物组的α多样性，但对微生物组组成有巨大影响。在吸烟组中，罗特氏菌属、放线菌属、嗜血杆菌属、卟啉单胞菌属、奈瑟菌属、不动杆菌属和链球菌属的相对丰度显著增加。另一方面，普氏菌属和韦荣球菌属的相对丰度显著下降。
吸烟可能会改变环境微生物，进而改变肺部微生物组的结构，从而导致与吸烟相关的疾病。
