Department of Chemistry, The University of Texas at Austin, 105 E 24th Street, Austin, TX 78712, USA.
Department of Molecular Biosciences, The University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA.
Angew Chem Int Ed Engl. 2022 Jun 13;61(24):e202202211. doi: 10.1002/anie.202202211. Epub 2022 Apr 29.
The use of DNA-based nanostructures as probes has led to significant advances in chemical and biological sensing, allowing the detection of analytes in complex media, the understanding of fundamental biological processes, and the ability to diagnose diseases based on molecular signatures. The utility of these structures arises both from DNA's inherent ability to selectively recognize and bind a variety of chemical species and from the unique properties observed when DNA is restructured at the nanoscale. In this Minireview, we chronicle the most commonly used signal transduction strategies that have been interfaced with various DNA-based nanostructures. We discuss the types of analytes and the detection scenarios that are sought after, delineate the advantages and disadvantages of each signaling strategy, and outline the key considerations that guide the selection of each signaling method.
DNA 纳米结构作为探针的应用在化学和生物传感领域取得了重大进展，使得在复杂介质中检测分析物、理解基本生物过程以及基于分子特征诊断疾病成为可能。这些结构的实用性源于 DNA 选择性识别和结合多种化学物质的固有能力，以及在纳米尺度上重新构建 DNA 时观察到的独特性质。在这篇综述中，我们记录了与各种 DNA 纳米结构接口的最常用的信号转导策略。我们讨论了所追求的分析物类型和检测场景，阐述了每种信号策略的优缺点，并概述了指导每种信号方法选择的关键考虑因素。
